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Researchers develop method to produce sweeter, well-growing tomatoes
6-7-2016 | Provided by: Tohoku University Japan

Posted on 06/07/2016 11:47:51 AM PDT by Red Badger

The researchers think it is likely that most flowering plants, or angiosperms, contain similar sucrose-susceptible genes, making their “sweetening technology” widely applicable. Credit: Cienpies Design

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Previous research has shown that the sugar sucrose plays a role in controlling key fruit genes involved in sugar metabolism. Efforts to control these genes succeeded in increasing the sugar content in fruit but also resulted in stunted growth.

Researchers from Tohoku University in Japan used a bioinformatics search tool to find nucleotide sequences in the tomato genome similar to a known tobacco gene sequence that can be repressed by sucrose. When a special coding sequence on the tobacco gene, called a uORF, is removed and the main sequence is made to overexpress, the gene activates several other genes involved in sugar metabolism, ultimately increasing sucrose levels in tobacco leaves. When the uORF is not removed, an overexpressing gene will increase sucrose content in the tobacco leaves but only up to a point. The increased sucrose represses the uORF, which in turn represses the main part of the gene, limiting its ability to further increase the fruit's sucrose content.

Two genes with very similar sequences to the tobacco gene were identified in the tomato plant by the researchers' bioinformatics search and their uORFs were removed. The main gene sequence was placed under the control of another gene, called E8, to express the target gene. The modified DNA was then inserted into tomato plants. The resulting tomato lines were found to have 50% more sucrose than normal tomatoes and showed no growth retardation. In addition to sugar metabolism, the tomato gene also affects genes involved in amino acid metabolism. The researchers found that levels of several amino acids were higher in the transgenic tomatoes.

Based on their results, the team is now developing a new modified DNA sequence containing 2A11, which functions during more of the fruit's development than E8, they say.

The researchers think it is likely that most flowering plants, or angiosperms, contain similar sucrose-susceptible genes, making their "sweetening technology" widely applicable.


TOPICS: Agriculture; Business/Economy; Food; Gardening
KEYWORDS: agriculture; gardening; sucrose; sugar; tobbaco; tomato
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To: Red Badger

We don’t need sweeter tomatoes.


21 posted on 06/07/2016 12:28:34 PM PDT by Brookhaven (Hillary Clinton stood next to the coffin of an American soldier and lied to his parents' face)
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To: Red Badger; greeneyes

Meh. I’m sweet enough, LOL!

Garden Ping to Greeneyes!


22 posted on 06/07/2016 12:31:10 PM PDT by Diana in Wisconsin (I don't have 'Hobbies.' I'm developing a robust Post-Apocalyptic skill set...)
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To: Red Badger

Sweet? That’s not a flavor that I’d associate with sliced, raw tomatoes. They’re supposed to be sort of tangy.


23 posted on 06/07/2016 12:33:33 PM PDT by RegulatorCountry
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To: Red Badger

“You’d better watch out
For the eggplant that ate Chicago
For he may come and eat your city soon”...

I don’t like the idea of GMOs-I grow my own tomatoes and other veggies and herbs-no inorganic pesticides, etc-I wouldn’t like a sweet tomato-the non-sweet, rich and sharp veggie flavor of a vine ripened tomato is like warm sunshine-and basil and other herbs and condiments blend with it so well...


24 posted on 06/07/2016 12:39:52 PM PDT by Texan5 ("You've got to saddle up your boys, you've got to draw a hard line"...)
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To: RegulatorCountry

The reason modern, mass produced foods are sweet is because they are devoid of other flavors.

Adding sugar is the food processing industry’s primary method to make bland food palatable.

What I take from this story is: “we grow lousy tasting tomatoes, so we’re going to add sugar to them to make them edible.”


25 posted on 06/07/2016 12:40:08 PM PDT by Brookhaven (Hillary Clinton stood next to the coffin of an American soldier and lied to his parents' face)
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To: Diana in Wisconsin

Thanks for the ping. I prefer heirloom tomatoes. Since Dr. only allows me 50g of Carbs per day plus all the greens such as lettuce I can stand, I don’t need a sweeter tomato.

With all the sugar laden food at the grocery, I suspect other people don’t either. Gave up all added sugar in 2009, along with soda. I drink unsweetened tea, unsweetened lemonade-don’t use artificial sweetener either.

Just gradually cut back, and taste buds adjusted. Can’t stand to drink a soda now - too sweet.

My favorite meal growing up: Pinto Beans, Corn, fried taters with ketchup, sliced home grown tomatoes, cornbread and loads of butter. High carbs - no doubt of it.

I agree and join you we are sweet enough! LOL


26 posted on 06/07/2016 12:48:20 PM PDT by greeneyes (Moderation in defense of your country is NO virtue. Le//t Freedom Ring.)
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To: Brookhaven

Agree. Commercial agriculture is concerned more with production than with taste. They could grow better-tasting varieties, but not if they grow more slowly or produce less fruit.


27 posted on 06/07/2016 12:50:27 PM PDT by Rio (Proud resident of the State of Jefferson)
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To: Red Badger

I miss the non-hybrid tomatoes that had acid in them that made pimples in your mouth by midsummer when you had eaten them every day for weeks.

Tomatoes don’t taste the same anymore. They are bred and selected for everything except how they taste.


28 posted on 06/07/2016 12:50:40 PM PDT by E. Pluribus Unum (If an illegal-alien quarantine saves just one child's life, it will be worth it.)
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To: Red Badger


29 posted on 06/07/2016 12:52:26 PM PDT by JoeProBono (SOME IMAGES MAY BE DISTURBING ’VIEWER DISCRETION IS ADVISED;-{)
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To: Red Badger

They can’t stop complaining that 1/3 of Americans are obese, because we eat too much sugar and not enough vegetables.

Now they want to put sugar in the vegetables?!

Because we’re not fat enough apparently?


30 posted on 06/07/2016 12:53:02 PM PDT by Black Agnes
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To: Black Agnes

A lot of places add way too much sugar to their tomato sauce.

It always amazes me when people buy their tomato sauce in cans or jars. It really doesn’t take long to make, and it tastes so much better.


31 posted on 06/07/2016 1:10:13 PM PDT by Vermont Lt (Ask Bernie supporters two questions: Who is rich. Who decides. In the past, that meant who died.)
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To: Black Agnes

http://www.princeton.edu/main/news/archive/S26/91/22K07/


32 posted on 06/07/2016 1:21:29 PM PDT by Red Badger (Make America AMERICA again!.........................)
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To: Red Badger

No thanx. I think I’ll just stick with my heritage seeds.


33 posted on 06/07/2016 1:22:50 PM PDT by Georgia Girl 2 (The only purpose of a pistol is to fight your way back to the rifle you should never have dropped)
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To: Red Badger

it’s just interesting that they’d take something considered fairly healthy...and add sugar.

As I said, apparently they don’t think we’re fat enough yet.


34 posted on 06/07/2016 1:24:14 PM PDT by Black Agnes
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To: Red Badger

There are PLENTY of ‘sweet’ tomatoes on the heirloom/open-pollinated market already. Breeders are adding more every year.


35 posted on 06/07/2016 1:26:50 PM PDT by who knows what evil? (Yehovah saved more animals than people on the ark...www.siameserescue.com)
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To: Black Agnes

Sugars in some form are added to processed foods so people will buy more of the unhealthy nutrition-poor crap-without anything added, packaged/processed food tastes like Styrofoam. Makes just as much sense as the stupid food pyramid-when they figured out it was wrong, they changed it-and it is still wrong-it emphasizes too many carbs-again...

I wasn’t brought up eating processed frankenfood, and I’m not going to eat in now unless I’m in danger of starvation...


36 posted on 06/07/2016 1:35:27 PM PDT by Texan5 ("You've got to saddle up your boys, you've got to draw a hard line"...)
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To: JoeProBono

That’s what they do here in East Tennessee...night before a frost in late fall; farmers send the illegals out into the fields to pick all the green tomatoes they can find. Then the unripe ‘maters are subjected to the gas on the truck so they are ‘ripe’ when they arrive at market. Enjoy.


37 posted on 06/07/2016 1:43:01 PM PDT by who knows what evil? (Yehovah saved more animals than people on the ark...www.siameserescue.com)
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To: Vermont Lt

There are very few store sauces that do not contain sugar, fructose, high fructose corn syrup, whatever...Classico has one variety that I have found that does NOT contain a sweetener...most others are very high end sauces (made by NY mobsters and so forth...with the ‘Hoffa meatballs...YUM!)


38 posted on 06/07/2016 1:47:04 PM PDT by who knows what evil? (Yehovah saved more animals than people on the ark...www.siameserescue.com)
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To: Red Badger

I don’t want tomatoes that taste like candy. Really, does EVERYTHING have to taste like candy? I guess diabetes causing tomatoes are unavoidable. grrr.


39 posted on 06/07/2016 1:53:56 PM PDT by pa_dweller (Of all the things I've lost I miss my mind the most.)
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To: JoeProBono

I used to haul tomatoes from Florida to California. We would stop at a state facility in Gainsville to change them from green to red in 30 minutes. They were the worst tasting tomatoes you would ever eat but Californians would buy them. They were rock hard, bitter, juiceless. Yuck but it paid the bills.


40 posted on 06/07/2016 4:18:56 PM PDT by B4Ranch (In a time of universal deceit, telling the truth is a revolutionary act.--Orwell)
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