Posted on 05/13/2025 4:37:24 AM PDT by Red Badger
Researchers have found that the source of protein in your diet may dramatically shape your gut microbiome, influencing key metabolic processes and even gut lining health. The results suggest certain proteins, like egg whites, could have surprising and possibly harmful effects on gut function.
================================================================================
New research from NC State reveals that different protein sources in animal diets can dramatically alter the gut microbiome, both in composition and function.
A new study from researchers at North Carolina State University has found that the type of protein in an animal’s diet significantly influences both the composition and activity of the gut microbiome, the community of microorganisms living in the digestive tract.
These gut microbes play a crucial role in various aspects of health, and the study’s findings could contribute to improved strategies for preventing and treating gastrointestinal diseases that affect millions of people worldwide.
“There’s something wrong with what we’re eating today, and we are not close to knowing what that is,” said Alfredo Blakeley-Ruiz, an NC State postdoctoral researcher and co-corresponding author of a paper describing the study. “Our lab wanted to know how different diets impact what lives in the gut, and to learn something about what those microbes are doing, functionally, in response to that diet.”
In their experiment, the researchers examined the effects of specific dietary protein sources, such as those from milk, eggs, and plants like peas and soy, on the gut microbiomes of mice. The mice were each fed a diet consisting of a single protein source for one week. These sources included egg whites, brown rice, soy, and yeast, allowing the researchers to isolate the impact of each protein on the gut microbial ecosystem.
Shifts in Microbial Populations and Activity
Using an integrated metagenomics-metaproteomics approach requiring high resolution mass spectrometry, the researchers found that the mice gut microbiome changed a lot over the course of the study, with some protein sources showing extreme effects.
“The composition of the gut microbiome significantly changed every time we changed the protein source,” Blakeley-Ruiz said. “The protein sources with the biggest functional effects were brown rice, yeast, and egg whites.”
In examining the functional changes in the gut microbiome, the study showed that the two largest effects of dietary protein were on amino acid metabolism, which was expected by the researchers, and complex sugar degradation, which was not.
Brown rice and egg whites increase amino acid degradation in the mouse gut microbiome.
“Brown rice and egg white diets increased amino acid degradation in the mouse gut microbiome, meaning that the microbes were breaking down those proteins instead of making their own amino acids from scratch,” Blakeley-Ruiz said. “This makes intuitive sense because proteins are made of amino acids, but this is something we want to dig into more. Some amino acids can degrade into toxins, and others can impact the gut-brain axis, so there are potential health implications from these diets.”
The study also showed that long chains of sugars attached to the dietary proteins, called glycans, also play a role in changing gut microbiome function. Multiple dietary protein sources, including soy, rice, yeast, and egg white, caused microbes in the gut to change the production of enzymes that break down glycans, sometimes substantially.
“This could be really meaningful, health wise,” Blakeley-Ruiz said. “In the egg white diet, in particular, one bacterium took over and activated a bunch of glycan-degrading enzymes. We then grew this bacterium in the lab and found that the glycan-degrading enzymes it produced in media containing egg white protein were similar to those produced in media containing mucin.”
Potential Implications for Gut Health
Mucin is the substance that lines the inside of the gut, protecting the digestive system from things like acid and pathogens. So if bacteria are producing enzymes that, purposely or not, break down mucin, they could be damaging the intestinal lining and causing negative impacts on gut health.
“I’m excited to explore this potential connection between the expression of glycan degrading enzymes in the egg white diet and the breakdown of mucin by the gut microbiome in future studies,” says Blakeley-Ruiz.
Manuel Kleiner, an NC State associate professor of plant and microbial biology and co-corresponding author of the paper, said the study lays the groundwork for future investigation of the effects of protein sources on the gut microbiome.
“One of the limitations of our study is that, of course, the diets are very artificial and could lead to amplified results,” Kleiner said. “But we now show that egg white has extreme effects on the microbiome. For the future, we’re very interested in understanding what the mechanism of this effect is in a mixed protein diet in mice.
“Our study shows not only which bacterial species are in the gut microbiome and their abundance, but also what they are actually doing. Here, they are specifically digesting the glycans. The result is a very comprehensive picture of what really matters in the gut in terms of diet and function.”
Reference:
“Dietary protein source alters gut microbiota composition and function”
by J Alfredo Blakeley-Ruiz, Alexandria Bartlett, Arthur S McMillan, Ayesha Awan, Molly Vanhoy Walsh, Alissa K Meyerhoffer, Simina Vintila, Jessie L Maier, Tanner G Richie, Casey M Theriot and Manuel Kleiner, 21 March 2025, The ISME Journal.
DOI: 10.1093/ismejo/wraf048
The study was supported by the National Institutes of Health through awards R35GM138362, T32DK007737 and P30 DK034987, and by the USDA National Institute of Food and Agriculture, Hatch project 7002782.
We now have a “connection” for fresh eggs. I get about two dozen per week and they are delicious.
I’m not a mouse. These “mouse” tests have zero credibility.
Wife and I eat eggs maybe three times a week. Much more worried about out driving on the interstate with illegals that the gut microbe of mice🐁
“it stays crunchy, even in milk.”
That crunch enhancer was invented by Clark W. Griswold
In a thousand years we will have created the healthiest super mice ever...............
Healthy Food Fear Porn
Screw their education. I will eat eggs as I like. I am healthy, and not buying the shit they try to call candy.
Sprayed with glyphosate because f#$% you guys and your health! Or so it is reported....
I eat four to six eggs per day. Docs are impressed with my health.
Who pays for a study often determines the results - just saying.
“Brown rice and egg whites increase amino acid degradation in the mouse gut microbiome.”
Then stop feeding your mice this combo! ;)
Another day, another study. We’re all gonna die.
“You will eat ze bugs.”
Flockov.
Egg and olive….now THERE’S a combination I hadn’t thought of! Thanks!😛
Whose money pays for this “research”? Looks like this one is just an excuse to beg for more research grant money to find out more.
Another food fad scare.
I remember my Sister-in-Law saying that in 1960 added gluten was all the rage.
Every few years they come out with something new. I remember when Acidophilis milk was all the rage and in all the stores. Now you cannot find it.
Then chicken skin was bad.
Egg yokes bad.
Beef bad.
Now it is egg whites and brown rice bad.
20 years ago they told us it was the egg yolks that were bad for you and recommended that you stick with only egg whites. Now they caution against egg whites. Who knows what they will be saying next week.
Spanish olives with pimento. I found a jar already sliced at a reasonable price.
Why is it they never come out with some study that says Broccoli and Asparagus is bad?...................
I do about the same. The only bad number I had was B12. I’m 81.
Yeh, sure. 🙄
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.