Posted on 01/08/2021 12:24:08 PM PST by Red Badger
Humans have them, so do other animals and plants. Now research reveals that bacteria too have internal clocks that align with the 24-hour cycle of life on Earth.
The research answers a long-standing biological question and could have implications for the timing of drug delivery, biotechnology, and how we develop timely solutions for crop protection.
Biological clocks or circadian rhythms are exquisite internal timing mechanisms that are widespread across nature enabling living organisms to cope with the major changes that occur from day to night, even across seasons.
Existing inside cells, these molecular rhythms use external cues such as daylight and temperature to synchronise biological clocks to their environment. It is why we experience the jarring effects of jet lag as our internal clocks are temporarily mismatched before aligning to the new cycle of light and dark at our travel destination.
A growing body of research in the past two decades has demonstrated the importance of these molecular metronomes to essential processes, for example sleep and cognitive functioning in humans, and water regulation and photosynthesis in plants.
Although bacteria represent 12% biomass of the planet and are important for health, ecology, and industrial biotechnology, little is known of their 24hr biological clocks.
Previous studies have shown that photosynthetic bacteria which require light to make energy have biological clocks.
But free-living non photosynthetic bacteria have remained a mystery in this regard.
In this international study researchers detected free running circadian rhythms in the non-photosynthetic soil bacterium Bacillus subtilis.
The team applied a technique called luciferase reporting, which involves adding an enzyme that produces bioluminescence that allows researchers to visualise how active a gene is inside an organism.
They focused on two genes: firstly, a gene called ytvA which encodes a blue light photoreceptor and secondly an enzyme called KinC that is involved in inducing formation of biofilms and spores in the bacterium.
They observed the levels of the genes in constant dark in comparison to cycles of 12 hours of light and 12 hours of dark. They found that the pattern of ytvA levels were adjusted to the light and dark cycle, with levels increasing during the dark and decreasing in the light. A cycle was still observed in constant darkness.
Researchers observed how it took several days for a stable pattern to appear and that the pattern could be reversed if the conditions were inverted. These two observations are common features of circadian rhythms and their ability to "entrain" to environmental cues.
They carried out similar experiments using daily temperature changes; for example, increasing the length or strength of the daily cycle, and found the rhythms of ytvA and kinC adjusted in a way consistent with circadian rhythms, and not just simply switching on and off in response to the temperature.
"We've found for the first time that non-photosynthetic bacteria can tell the time," says lead author Professor Martha Merrow, of LMU (Ludwig Maximilians University) Munich. "They adapt their molecular workings to the time of day by reading the cycles in the light or in the temperature environment."
"In addition to medical and ecological questions we wish to use bacteria as a model system to understand circadian clock mechanisms. The lab tools for this bacterium are outstanding and should allow us to make rapid progress," she added.
This research could be used to help address such questions as: is the time of day of bacterial exposure important for infection? Can industrial biotechnological processes be optimised by taking the time of day into account? And is the time of day of anti-bacterial treatment important?
"Our study opens doors to investigate circadian rhythms across bacteria. Now that we have established that bacteria can tell the time we need to find out the processes that cause these rhythms to occur and understand why having a rhythm provides bacteria with an advantage," says author Dr. Antony Dodd from the John Innes Centre.
Professor Ákos Kovács, co-author from the Technical University of Denmark adds that "Bacillus subtilis is used in various applications from laundry detergent production to crop protection, besides recently exploiting as human and animal probiotics, thus engineering a biological clock in this bacterium will culminate in diverse biotechnological areas."
The study appears in Science Advances.
More information: A circadian clock in a non-photosynthetic prokaryote, Science Advances (2021). advances.sciencemag.org/lookup … .1126/sciadv.abe2086 Journal information: Science Advances
https://advances.sciencemag.org/lookup/doi/10.1126/sciadv.abe2086
Does anybody really care?
They need to know when happy hour is.
If so, I can’t imagine why......................
Yeah, but they can’t judge distance. Ever see one try toss a free throw?
It’s always 5 O’Clock somewhere.
Yes, but can they chew gum and whistle Dixie at the same time? That is the important question.
Seriously though, Cool article
which is more than many D congress-critters can do
Sometimes they have a party in my gut in the middle of the night.................
Bacteria may, but millennials need a digital clock to do that.
You ain’t kidding!
My son-in-law’s niece, 18 years old, cannot tell time on an analog clock!.........................
No
So bacteria is is a cognizant entity?
That’s why they have tiny little watches.
I can’t remember if I learned that in school, my parents taught me, or I just picked it up.
But I’m pretty sure that I knew by first grade. Because every classroom had an analog clock on the wall, and you were always interested in knowing when school got out at 3:00 p.m.
Of course they can. They know precisely since I had my last cold, that I’m overdue for one, and then they amass for the attack.
The old analog school clocks that twitched every minute taught me how time works. They slowed WAY down when the teacher was droning on, sped way up during recess and lunch, and almost slowed to a stop the hour before school was over. Time stopped entirely when Karen sent a brief glance my way, too.
I learned time was very elastic.
That is so true.
And do you remember, on the last day before summer vacation. When it got to be about 2:30 the clock seemed to go so slow. Sometimes it even seem to be going backwards.
how do they celebrate New Year?
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