Posted on 06/17/2020 12:03:38 AM PDT by Grandpa Drudge
From the first paragraph of this article:
“This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans.”
And from the results summary in this article:
Using the SARS-CoV infectious clone as a template (7), we designed and synthesized a full-length infectious clone of WIV1-CoV consisting of six plasmids that could be enzymatically cut, ligated together, and electroporated into cells to rescue replication competent progeny virions (Fig. S1A). In addition to the full-length clone, we also produced WIV1-CoV chimeric virus that replaced the SARS spike with the WIV1 spike within the mouse-adapted backbone (WIV1-MA15, Fig. S1B). WIV1-MA15 incorporates the original binding and entry capabilities of WIV1-CoV, but maintains the backbone changes to mouse-adapted SARS-CoV. Importantly, WIV1-MA15 does not incorporate the Y436H mutation in spike that is required for SARS-MA15 pathogenesis (8). Following electroporation into Vero cells, robust stock titers were recovered from both chimeric WIV1-MA15 and WIV1-CoV.
(Excerpt) Read more at pnas.org ...
Here is the blueprint for Modifying (man made) SARS-Cov based Virus
Published in PNAS (Proceedings of the National Academy of Sciences of the United Strates of America) March 14, 2016
https://www.pnas.org/content/113/11/3048
SARS-like WIV1-CoV poised for human emergence
From the first paragraph of this article:
“This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans.”
And from the results summary in this article:
Using the SARS-CoV infectious clone as a template (7), we designed and synthesized a full-length infectious clone of WIV1-CoV consisting of six plasmids that could be enzymatically cut, ligated together, and electroporated into cells to rescue replication competent progeny virions (Fig. S1A). In addition to the full-length clone, we also produced WIV1-CoV chimeric virus that replaced the SARS spike with the WIV1 spike within the mouse-adapted backbone (WIV1-MA15, Fig. S1B). WIV1-MA15 incorporates the original binding and entry capabilities of WIV1-CoV, but maintains the backbone changes to mouse-adapted SARS-CoV. Importantly, WIV1-MA15 does not incorporate the Y436H mutation in spike that is required for SARS-MA15 pathogenesis (8). Following electroporation into Vero cells, robust stock titers were recovered from both chimeric WIV1-MA15 and WIV1-CoV.
And the connection to Wuhan Labs in China is highlighted in the Acknowledgments:
We thank Dr. Zhengli-Li Shi of the Wuhan Institute of Virology for access to bat CoV sequences and plasmid of WIV1-CoV spike protein. Research was supported by the National Institute of Allergy and Infectious Disease and the National Institute of Aging of the NIH under Awards U19AI109761 and U19AI107810 (to R.S.B.), AI1085524 (to W.A.M.), and F32AI102561 and K99AG049092 (to V.D.M.). Human airway epithelial cell cultures were supported by the National Institute of Diabetes and Digestive and Kidney Disease under Award NIH DK065988 (to S.H.R.). Support for the generation of the mice expressing human ACE2 was provided by NIH Grants AI076159 and AI079521 (to A.C.S.).
And an interesting side note: National Institute of Allergy and Infectious Disease (NIAID) is managed by Dr. Anthony Fauci, in case you were wondering if he had any connection to this.
My assessment is that the PNAS article indicates strongly that NIH National Institute of Allergy and Infectious Disease, managed br Dr. Anthony Fauci, and the Wuhan Institute of Virology, managed by Dr. Zhengli-Li Shi cooperated to create what ultimately became the chimeric virus SARS-CoV-2 (Covid-19) pandemic.
https://www.pnas.org/content/113/11/3048
My further assessment is that the gop-foreignaffairs report is investigating CPP for malfeasence, but apparently not interested in understanding where this virus originated, they are only interested in blaming CCP for the cover up.
Bttt.
5.56mm
“Chimeric virus”...I am reminded of “Chimera” in MISSION IMPOSSIBLE 2...It was a biological weapon...
MI 2 was a good action film...
There was an article posted to free republic maybe a month ago, from a scientist saying that this sort of thing was well known in the scientific community -— that they had been researching this modification of corona viruses...
But for whatever reason the press isn’t reporting it.
Yeah... it isn’t conspiracy talk any more
If it waddles like Peking duck...
Title is:
SARS-like WIV1-CoV poised for human emergence
Not the title you gave it.
You don’t know anything about molecular biology or bioinformatics, do you?
“SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus”
I don’t see anything in the paper saying that.
In fact it expressly says that SARS-CoV likely emerged from Chinese horseshoe bats, and that similar viruses living in that bat population could emerge as future SARS outbreaks.
The paper details stupid virology tricks, but does not say covid 19 was man-made.
Posting fake headlines especially while a noob is not a promising way start on Free Republic.
The Chinese have a cure. Make them give it to us.
DON'T POST LIES.
“The Chinese have a cure. Make them give it to us.”
How do you propose to do that? Trying to use force would be worse than the disease.
Re: “Not the title you gave it.”
Besides the harmless “[2016],” the title looks exact to me.
Looks like you stirred up a severely concerned set of “Karens”...
First for kindly letting us know it was a 2016 article,
then for making some reasonable observations,
and for highlighting connections contained within.
You must be over the target.
Thanks for posting!
Comment #1 is not accurate.
However, links to this paper have been posted several times at Free Republic, and it does contain important information.
The Wuhan Lab, and specifically its director, were attempting to modify the bat virus into a form that would infect humans, a form that could be studied in the lab before a natural transition might take place.
The Wuhan Lab director's work was funded by NIAID, where Dr. Fauci is the director. Presumably, Dr. Fauci approved the Wuhan funding.
Since COVID-19 was first identified at an open market place within walking distance of the Wuhan Lab, and since Dr. Fauci was a top USA government executive involved in the COVID-19 crisis, that is definitely a coincidence worth investigating.
Naturally, none of the mainstream press will investigate, so the amateurs at Free Republic - like me - have to stumble around on our own.
“Besides the harmless “[2016],” the title looks exact to me.”
Admins changed it to the correct title from the listed fake title.
It was initially posted as “SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus”
is the title I intended to use.
The title that appears is the title of the PNAS article that serves as the basis of this claim.
I am not an expert at FR posts, and don't know why FR used the article title rather than the title I specified.
Looks the same to me, with the helpful date flag.
I'm certainly not expert in those fields, but I DO know how to read. Do you?
There is plenty of plainly stated information in that Proceedings of the National Academy of Sciences article to support my assessment.
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