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Mitochondrial DNA Mutation Rates
University of North Carlolina Computer Science Department website ^ | from 1997 to present | David Plaistid

Posted on 09/01/2002 4:20:09 PM PDT by Ahban

Mitochondrial DNA Mutation Rates

David A. Plaisted

Recently an attempt was made to estimate the age of the human race using mitochondrial DNA. This material is inherited always from mother to children only. By measuring the difference in mitochondrial DNA among many individuals, the age of the common maternal ancestor of humanity was estimated at about 200,000 years. A problem is that rates of mutation are not known by direct measurement, and are often computed based on assumed evolutionary time scales. Thus all of these age estimates could be greatly in error. In fact, many different rates of mutation are quoted by different biologists.

It shouldn't be very hard explicitly to measure the rate of mutation of mitochondrial DNA to get a better estimate on this age. From royal lineages, for example, one could find two individuals whose most recent common maternal ancestor was, say, 1000 years ago. One could then measure the differences in the mitochondrial DNA of these individuals to bound its mutation rate. This scheme is attractive because it does not depend on radiometric dating or other assumptions about evolution or mutation rates. It is possible that in 1000 years there would be too little difference to measure. At least this would still give us some useful information.

(A project for creation scientists!)

Along this line, some work has recently been done to measure explictly the rate of substitution in mitochondrial DNA. The reference is Parsons, Thomas J., et al., A high observed substitution rate in the human mitochondrial DNA control region, Nature Genetics vol. 15, April 1997, pp. 363-367. The summary follows:

"The rate and pattern of sequence substitutions in the mitochondrial DNA (mtDNA) control region (CR) is of central importance to studies of human evolution and to forensic identity testing. Here, we report a direct measurement of the intergenerational substitution rate in the human CR. We compared DNA sequences of two CR hypervariable segments from close maternal relatives, from 134 independent mtDNA lineages spanning 327 generational events. Ten subsitutions were observed, resulting in an empirical rate of 1/33 generations, or 2.5/site/Myr. This is roughly twenty-fold higher than estimates derived from phylogenetic analyses. This disparity cannot be accounted for simply by substitutions at mutational hot spots, suggesting additional factors that produce the discrepancy between very near-term and long-term apparent rates of sequence divergence. The data also indicate that extremely rapid segregation of CR sequence variants between generations is common in humans, with a very small mtDNA bottleneck. These results have implications for forensic applications and studies of human evolution." (op. cit. p. 363).

The article also contains this section: "The observed substitution rate reported here is very high compared to rates inferred from evolutionary studies. A wide range of CR substitution rates have been derived from phylogenetic studies, spanning roughly 0.025-0.26/site/Myr, including confidence intervals. A study yielding one of the faster estimates gave the substitution rate of the CR hypervariable regions as 0.118 +- 0.031/site/Myr. Assuming a generation time of 20 years, this corresponds to ~1/600 generations and an age for the mtDNA MRCA of 133,000 y.a. Thus, our observation of the substitution rate, 2.5/site/Myr, is roughly 20-fold higher than would be predicted from phylogenetic analyses. Using our empirical rate to calibrate the mtDNA molecular clock would result in an age of the mtDNA MRCA of only ~6,500 y.a., clearly incompatible with the known age of modern humans. Even acknowledging that the MRCA of mtDNA may be younger than the MRCA of modern humans, it remains implausible to explain the known geographic distribution of mtDNA sequence variation by human migration that occurred only in the last ~6,500 years.

One biologist explained the young age estimate by assuming essentially that 19/20 of the mutations in this control region are slightly harmful and eventually will be eliminated from the population. This seems unlikely, because this region tends to vary a lot and therefore probably has little function. In addition, the selective disadvantage of these 19/20 of the mutations would have to be about 1/300 or higher in order to avoid producing more of a divergence in sequences than observed in longer than 6000 years. This means that one in 300 individuals would have to die from having mutations in this region. This seems like a high figure for a region that appears to be largely without function. It is interesting that this same biologist feels that 9/10 of the mutations to coding regions of DNA are neutral. This makes the coding regions of DNA less constrained than the apparently functionless control region of the mitochondrial DNA!


TOPICS: Miscellaneous
KEYWORDS: creationsim; crevolist; dna; evolution; godsgravesglyphs; helixmakemineadouble; history; mtdna; multiregionalism; mutationrates
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To: Kyrie
I liked that. Good summation!
121 posted on 09/04/2002 5:29:15 PM PDT by Tribune7
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To: VadeRetro
Give me 24 more hours on Mungo Man and Qazfeh. I hope that I will not need it to write a concession speech, the first to you in over a year.
122 posted on 09/04/2002 5:58:18 PM PDT by Ahban
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To: VadeRetro
(Crash!)

OMG! The three commandments of evolution.

123 posted on 09/04/2002 6:08:03 PM PDT by Nebullis
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Five, that is. (I can't count.)
124 posted on 09/04/2002 6:15:43 PM PDT by Nebullis
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To: Kyrie
No candy?
125 posted on 09/04/2002 6:24:45 PM PDT by <1/1,000,000th%
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To: Ahban
Give me 24 more hours on Mungo Man and Qazfeh. I hope that I will not need it to write a concession speech, the first to you in over a year.

Since you can have as long as you want whether I give it to you or not, I might as well say, "You can have as long as you want." ;)

126 posted on 09/04/2002 6:35:12 PM PDT by VadeRetro
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To: Ahban
A lot of naysaying on Mungo Man here.
127 posted on 09/04/2002 6:54:01 PM PDT by VadeRetro
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To: Ahban
A Page on Early Modern Homo Sapiens with Neanderthal differences.
128 posted on 09/04/2002 7:06:53 PM PDT by VadeRetro
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To: Hunble
Along this line, some work has recently been done to measure explictly the rate of substitution in mitochondrial DNA. The reference is Parsons, Thomas J., et al., A high observed substitution rate in the human mitochondrial DNA control region, Nature Genetics vol. 15, April 1997, pp. 363-367. The summary follows: As new information is learned, one of use will end up looking like a fool.

Yes, the evolutionists are the fools. The article above is not about assumptions, it is about scientific research. Something which the evolutionists have never done in dozens of years in which they have been blaberring about mitochondrial DNA proving evolution. Facts beat rhetoric. Models based on no research are garbage. This is what the article proves. Evolutionists just make up garbage models based solely on what they want to believe, there is a phrase that fits perfectly such enterprises, but it cannot be said in a forum like this. When evolutionists start doing real research instead of building models based on nothing, then they can talk. The facts are below, from the article. You guys have no facts, just wishful thinking:

"The rate and pattern of sequence substitutions in the mitochondrial DNA (mtDNA) control region (CR) is of central importance to studies of human evolution and to forensic identity testing. Here, we report a direct measurement of the intergenerational substitution rate in the human CR. We compared DNA sequences of two CR hypervariable segments from close maternal relatives, from 134 independent mtDNA lineages spanning 327 generational events. Ten subsitutions were observed, resulting in an empirical rate of 1/33 generations, or 2.5/site/Myr. This is roughly twenty-fold higher than estimates derived from phylogenetic analyses. This disparity cannot be accounted for simply by substitutions at mutational hot spots, suggesting additional factors that produce the discrepancy between very near-term and long-term apparent rates of sequence divergence. The data also indicate that extremely rapid segregation of CR sequence variants between generations is common in humans, with a very small mtDNA bottleneck. These results have implications for forensic applications and studies of human evolution." (op. cit. p. 363).

129 posted on 09/04/2002 8:56:24 PM PDT by gore3000
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To: Nebullis
They mean to ask for evidence that they want to believe. I'll hunt up some models written on stone tablets.

Wrong, we ask for evidence for what you call science, you give us none. When you see real evidence from work done by real scientists who look at the facts not at your wishful thinking, you say it does not count, you say it does not matter, you say that whatever does not fit your stupid theory is false. Those are the actions and mode of thinking of ideologues, not people interested in science, in the truth or in what is real. If all we had was evo scientists in this world, we would be back in the stone age in no time at all.

130 posted on 09/04/2002 9:01:07 PM PDT by gore3000
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To: VadeRetro
From the other link, wonder why you a lover of skulls did not show us this one:

and

Another great example of paleontological nonsense. Oh, and BTW, in another article here the mtDNA nonsense on which the dating for whatever this is is thoroughly debunked.

131 posted on 09/04/2002 9:15:44 PM PDT by gore3000
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To: gore3000
RTFT. (Read the thread!)
132 posted on 09/05/2002 6:14:53 AM PDT by VadeRetro
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To: Ahban
Neanderthal-Early Modern Hybrid Found in Portugal. (Sciam, April 2000.)

How much does it help you that Neanderthals are "offline" if they're so related they can mate with humans? That's evidence for the "same species" classification, Homo sapiens neandertalensis. It goes back and forth between that and Homo neandertalensis. Can you connect dots, even when the pattern isn't what you prefer to believe?


133 posted on 09/05/2002 8:09:30 AM PDT by VadeRetro
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To: VadeRetro
RTFT. (Read the thread!)

Better save that one. You'll need to use it often.

134 posted on 09/05/2002 9:54:54 AM PDT by balrog666
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To: balrog666
You'll need to use it often.

Only as often as I bother. (Grumble! Grumble!)

135 posted on 09/05/2002 10:59:10 AM PDT by VadeRetro
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To: VadeRetro
Horses and donkies are in the same family, but otherwise not that closely related. They can interbreed, but almost never produce fertile offspring.

I am not sure what is being discussed in Genesis 6, but it does seem like the Bible refers to something, demons, other bipedal primates, whatever, that did interbreed with humans. Since what I am defending is the Bible, I can't close the door to the idea that God created more than one kind. Its in the Book. I don't want to defend just one nattow interpretatioin of the book, but adjust my interpretaion when in truth it needs it.

Are naturalists willing to do the same?

136 posted on 09/05/2002 2:06:27 PM PDT by Ahban
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To: Ahban
Somebody thought Neanderthals were demons? Maybe! That doesn't mean I have to think so.
137 posted on 09/05/2002 2:12:05 PM PDT by VadeRetro
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To: Ahban
Horses and donkies are in the same family, but otherwise not that closely related. They can interbreed, but almost never produce fertile offspring.

Horses and donkeys are just barely different species. Not only the same family but the same genus. Equus caballus versus equus asinus.

138 posted on 09/05/2002 2:19:26 PM PDT by VadeRetro
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To: Ahban; Condorman
A fun web page on known hybrids pointed out to me by Condorman a day or two back. That these things can happen indicates that the parent species involved are not far diverged (and in some cases, like the coydog, not really diverged enough to be separate species.)
139 posted on 09/05/2002 2:23:14 PM PDT by VadeRetro
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To: VadeRetro
No, somebody, a lot of people, think the Nephilim and the 'sons of God' referenced in Genesis 6 were demons. Some say they were just men of high rank in the society. A few wonder if they were not other hominids. Ones with big, cunning brains but no true empathatic sense. No true sense of devotion to God, and completely without alturism. A race where what we sould define as a 'sociopath' was normal. In fact, they would have had no capacity to be anything but a sociopath by our standards. It just one hypothesis.

I don't know what they were, but the Bible does make a cryptic reference to one or more sets of beings who took human females as their own, and children were born as a result of the unions.



That was a good catch on Mungo Man. Sporting of you to put it up and save me the trouble of (probably) finding it.

I have done some things with the pictures of Quazfeh that have forced me to concede that the skull you showed should not be grouped with neadertals, even progressives. At worst, it looks intermediate between them and moderns. The brain case actually looks a bit larger than ours.

This one has actually forced me to pick up the hotline to RTB and put in a call for help. They should respond tomorrow.
140 posted on 09/05/2002 7:52:10 PM PDT by Ahban
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