The four letters of life are being added to.
Posted on 02/24/2004 3:55:22 AM PST by PatrickHenry
Guided evolution and HIV help create man-made stuff of life.
Researchers have found new ways to string together artificial DNA bases. The techniques could aid the creation of altered genetic material for applications in medicine and technology.
Floyd Romesberg and co-workers at the Scripps Research Institute in La Jolla, California, have harnessed the principles of evolution to find an enzyme capable of assembling non-standard DNA1.
In a second study, Steven Benner of the University of Florida in Gainesville and colleagues used an enzyme made by HIV, the virus that causes AIDS, to do the same job. Benner's enzyme is even capable of making multiple copies of non-natural DNA, opening up the possibility that the code could eventually evolve on its own2.
All natural DNA is made up of just four bases, but researchers have created non-natural bases. These can be used to make forms of DNA that are more robust than the natural kind and do not break apart when exposed to high temperatures. Such super-DNA could be useful in a wide range of medical and technological applications.
Stitched up
The tricky part is stitching these artificial bases into a chain, and getting the chains to replicate as natural DNA does. An enzyme called DNA polymerase does the job in nature.
DNA polymerase works its way along a template strand of DNA, reading each base and collecting a matching base to create a new, copied strand. But the enzyme turns up its nose at artificial bases.
By systematically tinkering with the structure of DNA polymerase at one or two specific locations, researchers can make enzymes that work with artificial bases. But this technique, called 'rational design', is a tedious and unpredictable process.
"Going the full distance is very difficult to do in a rational way," says Rui Sousa of the University of Texas Health Science Center at San Antonio. Sousa has designed polymerases that are being used commercially.
Romesberg and his co-workers tried a different approach called 'directed evolution'. They made millions of mutant polymerases by randomly scrambling part of the natural enzyme's chemical structure. Most of the mutants were useless, but some were quite good at stitching together non-standard bases. They plucked these useful mutants out of the crowd, and repeated the mutation and selection process to fine-tune their abilities.
After four rounds of selection, they found several mutants capable of doing the job. One was particularly good: it was able to copy the sequence of a template molecule into the modified form of DNA as efficiently and faithfully as DNA polymerase working with natural bases.
Carbon copies
But the mutant is not perfect. Like most modified polymerases, Romesberg's top candidate runs out of steam and stops working after adding five artificial bases to a growing chain.
Benner's team created a more successful polymerase by starting with a different enzyme altogether - a reverse transcriptase (RT) that is made by the HIV-1 form of the virus that causes AIDS. This RT mutates when the virus is hit with anti-HIV drugs. Benner's group checked mutated forms to find a modified RT capable of stitching two non-standard bases into strings of DNA. They fine-tuned this enzyme using rational design.
Their enzyme even works in a process called the polymerase chain reaction (PCR) , which is often used in biotechnology to make copies of short strands of DNA. Most constructed polymerases fail when researchers try to make multiple copies of artificial DNA using PCR; after several rounds of copying, imperfect polymerases start to weed out non-standard DNA. But Benner's enzyme does not seem to have this problem.
The four letters of life are being added to.
This involves promising lab results, and practical benefits too. What are the anti-evos gonna do now?
Does it really matter what they say or do?
It's best to just ignore them.
They've been left behind.
What they always do and move the goalpost.
You can't create a cat from scratch in laboratory now can you?
I hope this stuff doesn't escape in viral form. Ouch!
I can create scratches from a cat, does that count?
All your rhetoric does is prove that evolutionism, whatever its scientific roots, has beeome as much a closed, unfalsifiable system in the minds of its vocal proponents as creationism is in the minds of its.
By systematically tinkering with the structure of DNA polymerase at one or two specific locations, researchers can make enzymes that work with artificial bases. But this technique, called 'rational design', is a tedious and unpredictable process.Hah! It's turning out that "intelligent design" IS variation and [some or other kind of] selection."Going the full distance is very difficult to do in a rational way," says Rui Sousa of the University of Texas Health Science Center at San Antonio. Sousa has designed polymerases that are being used commercially.
Romesberg and his co-workers tried a different approach called 'directed evolution'. They made millions of mutant polymerases by randomly scrambling part of the natural enzyme's chemical structure. Most of the mutants were useless, but some were quite good at stitching together non-standard bases. They plucked these useful mutants out of the crowd, and repeated the mutation and selection process to fine-tune their abilities.
That is precisely the Darwinian model.
Worth repeating! I especially liked the little addition to the title of the article.
Also from the original article (sub-title):
Guided evolution and HIV help create man-made stuff of life.
Has there ever been anything *but* guided "evolution"?
If you're going to try to "create life" in a lab, you should start with your own material - not God's.
When scientists create something from nothing, I'll be the first in line to praise them!
ampu
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