Dietary pyruvate targets cytosolic phospholipase A2 to mitigate inflammation and obesity in mice

Medical Xpress / Higher Education Press / Protein & Cell ^ | July 17, 2024 | Sadaf Hasan et al

[ConservativeMind]

An article discusses the multifactorial nature of obesity, highlighting its association with chronic low-grade inflammation, or meta-inflammation, which contributes to metabolic disorders.

Pyruvate was found to inhibit adipogenic differentiation in vitro and significantly prevent HFD-induced weight gain and inflammation in mice.

The study underscores pyruvate's potential as a therapeutic option for obesity, given its previous clinical use and safety.

Key findings from the study include:

Pyruvate reduced intracellular lipid and triglyceride accumulation, indicating its effectiveness in suppressing adipogenesis across different cell types.

In vivo experiments with male mice indicated that pyruvate administration significantly prevented HFD-induced weight gain and central adiposity. Pyruvate-treated mice exhibited lower body weights and reduced abdominal circumferences compared to untreated HFD-fed mice. This was accompanied by a decrease in white adipose tissue (WAT) inflammation and metabolic dysregulation.

The study identified cPLA2 as a novel target of pyruvate using drug affinity responsive target stability, proteomics, and cellular thermal shift assays. cPLA2 plays a critical role in adipocytic differentiation and chronic inflammation.

Reduction of Inflammatory Markers: Pyruvate administration resulted in the downregulation of various inflammatory genes, including IL-6, IL-1B, CCL2, and TNFα. The study showed pyruvate's anti-inflammatory effects were mediated through the inhibition of cPLA2, which in turn reduced the NF-κB-dependent expression of pro-inflammatory cytokines.

The study establishes pyruvate as a promising therapeutic agent against obesity and its related inflammatory and metabolic disorders. By targeting cPLA2, pyruvate effectively inhibits adipogenesis, reduces WAT inflammation, and prevents HFD-induced weight gain.

These findings highlight the potential of pyruvate as an anti-inflammatory and anti-obesity supplement that could be integrated into regular diets or used as a dietary supplement.

Given its previous clinical use, pyruvate presents a safe and viable alternative for obesity treatment. The study's insights into the molecular mechanisms of pyruvate provide a basis for development of pyruvate-based interventions for metabolic diseases.

[ConservativeMind]

Pyruvate is available as a supplement and is found in a few foods, like red apples, which may have up to 1/2 gram, I noticed.

[ConservativeMind]

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[ganeemead]

One common side effect of pyruvic acid is fearful cases of hives. There are better ways to lose weight.

[BipolarBob]

I put out treadmills and dietary videos for my mice and rats and they ignore them. They just like to eat fast food and the brake lines on my SUV. I don’t think they like me very much.

[Gahanna Bob]

Weight loss is difficult for me. I definitely have metabolic syndrome and pre-diabetic. Do you have any advice?

[Flaming Conservative]

What is HFD induced weight gain?

[Delta 21]

Whoa, whoa, whoa! Way to many big words in this article. Gonna make my brain hurt!

[packagingguy]

High fructose diet.

Before congress passed sugar price supports in the 1980s Americans used sucrose.

Fructose bypasses the body’s satiety signaling so people and animals that eat a lot of it become fat. Sucrose doesn’t do this.

[BipolarBob]

If you’re pre-diabetic means you’re not on insulin yet. Get one of Jason Fungs books or watch his you-tube videos. Intermittent fasting is the way to go. Try different diets - keto, carnivore, low carb and of course fasting. Jason Fung gives helpful hints on fasting so you don’t get scared or frustrated. And how to do it safely.

[BipolarBob]

Chronic High Fat Diet. You’re supposed to know every obscure and archaic and medical acronym out there.

[Gahanna Bob]

Thanks. I do have Jason Fung’s book, The Obesity Code. I only read the first few pages a year ago. I have it in front of me now.

[ganeemead]

Simplest for many people is to cut sugar soft drinks out of your diet. Walmart has generic sucralose which is not expensive... That and ice tea or some powdered soft drink.