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Gene transcripts from ancient wolf analyzed after 14,000 years in permafrost
EurekAlert! ^ | July 30, 2019 | PLOS

Posted on 08/03/2019 10:48:05 PM PDT by SunkenCiv

RNA -- the short-lived transcripts of genes -- from the "Tumat puppy", a wolf of the Pleistocene era has been isolated, and its sequence analyzed in a new study by Oliver Smith of the University of Copenhagen and colleagues publishing on July 30 in the open-access journal PLOS Biology. The results establish the possibility of examining a range of RNA transcripts from ancient organisms, a possibility previously thought to be extremely unlikely because of the short lifespan of RNA.

DNA, which encodes the "hard copy" of genes, is known to survive for thousands of years under favourable conditions. But RNA -- the short-lived working copy of a gene, which is transcribed from DNA in the cell and forms the instructions for making proteins -- is rapidly broken down in living tissue by a suite of recycling enzymes. That instability typically continues after death, and because of that, researchers have generally assumed that the likelihood of finding intact an ancient cell's complement of RNA -- its transcriptome -- was vanishingly small. But there have been a few exceptions, mostly in plants, which led the authors to ask whether there might be ancient animal transcriptomes well-preserved enough to be sequenced.

They isolated and analyzed RNA from liver tissue of a 14,300-year-old canid, possibly a wolf or partially domesticated wolf-like creature, that had been preserved in Siberian permafrost until its discovery, as well as tissue from two 19th- and 20th-century wolves for comparison. Using a variety of transcriptomic techniques and quality control measures, the team showed that the RNA sequenced from the Pleistocene-era canid was truly representative of the animal's RNA, with many liver-specific transcripts that matched more modern samples from both wolves and dogs.

(Excerpt) Read more at eurekalert.org ...


TOPICS: History; Science; Travel
KEYWORDS: ancientautopsies; catastrophism; godsgravesglyphs; helixmakemineadouble; permafrost; pleistocene; pupcicle; pupcicles; pupsicle; pupsicles; rna; tumatdog; tumatpuppy
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To: riverrunner
"We have firearms big enough to take care of both of them."

Some people will only get one shot though.


41 posted on 08/05/2019 6:44:23 AM PDT by Rebelbase
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To: Rebelbase

Your turn to make a horrible pun, taiga, you’re it!


42 posted on 08/05/2019 6:46:46 AM PDT by null and void (When the only tool you have is a hammer, ALL your problems look like skulls.)
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To: Rebelbase

I canine understand why you’d say that...

http://www.freerepublic.com/focus/bloggers/3769218/posts?page=34#34


43 posted on 08/05/2019 7:21:11 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: Rebelbase

I canine understand why you’d say that...

http://www.freerepublic.com/focus/bloggers/3769218/posts?page=34#34


44 posted on 08/05/2019 7:21:48 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: null and void

I concur but I’m out of ideas.


45 posted on 08/05/2019 7:48:08 AM PDT by Rebelbase
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To: Rebelbase

What’s heavier, a gram of water, or a gram of butane?


46 posted on 08/05/2019 7:53:39 AM PDT by null and void (When the only tool you have is a hammer, ALL your problems look like skulls.)
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To: SunkenCiv

47 posted on 08/05/2019 8:05:17 AM PDT by Rebelbase
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To: null and void

African or European?


48 posted on 08/05/2019 8:05:53 AM PDT by Rebelbase
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To: Rebelbase
That's an old favorite. I hear they *pound*ed it out in just six weeks, working on location in Yugoslavia.

49 posted on 08/05/2019 8:41:44 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: Rebelbase
That's an old favorite. I hear they *pound*ed it out in just six weeks, working on location in Yugoslavia.

50 posted on 08/05/2019 8:42:11 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: Rebelbase
I think Plato discussed all this in the Phaedo.

51 posted on 08/05/2019 8:44:24 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv

That’s paw-sible.


52 posted on 08/05/2019 10:44:38 AM PDT by Rebelbase
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To: Rebelbase
It's the leash of our worries, though.

53 posted on 08/05/2019 10:59:36 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv

Wonderful!


54 posted on 08/05/2019 11:06:29 AM PDT by trisham (Zen is not easy. It takes effort to attain nothingness. And then what do you have? Bupkis.)
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To: trisham

Couple of pupsicles. Wish I'd thought of that...
Flight of the Conchords Ep 12 'Doggy Bounce'

Flight of the Conchords Ep 12 'Doggy Bounce'

55 posted on 08/05/2019 11:08:59 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv

LOL! Love it.


56 posted on 08/05/2019 11:17:09 AM PDT by trisham (Zen is not easy. It takes effort to attain nothingness. And then what do you have? Bupkis.)
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To: SunkenCiv

Yeah, you don’t want to take it that fur.


57 posted on 08/05/2019 11:18:44 AM PDT by Rebelbase
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To: Rebelbase
Sometimes I kennel help it.

58 posted on 08/05/2019 11:28:16 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv

I wonder howl long it’ll be before the clone it?


59 posted on 08/05/2019 11:38:59 AM PDT by Rebelbase
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To: Rebelbase
African or European?

Water or butane.

I find your first answer hard to swallow.

60 posted on 08/05/2019 11:58:28 AM PDT by null and void (When the only tool you have is a hammer, ALL your problems look like skulls.)
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