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Gene transcripts from ancient wolf analyzed after 14,000 years in permafrost
EurekAlert! ^ | July 30, 2019 | PLOS

Posted on 08/03/2019 10:48:05 PM PDT by SunkenCiv

RNA -- the short-lived transcripts of genes -- from the "Tumat puppy", a wolf of the Pleistocene era has been isolated, and its sequence analyzed in a new study by Oliver Smith of the University of Copenhagen and colleagues publishing on July 30 in the open-access journal PLOS Biology. The results establish the possibility of examining a range of RNA transcripts from ancient organisms, a possibility previously thought to be extremely unlikely because of the short lifespan of RNA.

DNA, which encodes the "hard copy" of genes, is known to survive for thousands of years under favourable conditions. But RNA -- the short-lived working copy of a gene, which is transcribed from DNA in the cell and forms the instructions for making proteins -- is rapidly broken down in living tissue by a suite of recycling enzymes. That instability typically continues after death, and because of that, researchers have generally assumed that the likelihood of finding intact an ancient cell's complement of RNA -- its transcriptome -- was vanishingly small. But there have been a few exceptions, mostly in plants, which led the authors to ask whether there might be ancient animal transcriptomes well-preserved enough to be sequenced.

They isolated and analyzed RNA from liver tissue of a 14,300-year-old canid, possibly a wolf or partially domesticated wolf-like creature, that had been preserved in Siberian permafrost until its discovery, as well as tissue from two 19th- and 20th-century wolves for comparison. Using a variety of transcriptomic techniques and quality control measures, the team showed that the RNA sequenced from the Pleistocene-era canid was truly representative of the animal's RNA, with many liver-specific transcripts that matched more modern samples from both wolves and dogs.

(Excerpt) Read more at eurekalert.org ...


TOPICS: History; Science; Travel
KEYWORDS: ancientautopsies; catastrophism; godsgravesglyphs; helixmakemineadouble; permafrost; pleistocene; pupcicle; pupcicles; pupsicle; pupsicles; rna; tumatdog; tumatpuppy
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To: PIF

https://gunwatch.blogspot.com/2019/03/pistols-or-handguns-95-effective-when.html

Yes I am sure I have large enough calibers.


21 posted on 08/04/2019 6:17:42 AM PDT by riverrunner ( o the public,)
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To: riverrunner

That’s in 95% of the cases against the slower and much smaller brown and black bears. As for the other 5% ... not to worry - it will take some time before the first Short Faced Bears are grown and released into the wild near you :)

Perhaps about that time they will release the first 30mm hand gun ...


22 posted on 08/04/2019 7:50:20 AM PDT by PIF (They came for me and mine ... now it is your turn ...)
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To: jimtorr; null and void; oldasrocks; PIF; riverrunner; Salamander; Sparticus; zeestephen
They found a mummified mailman with the seat of his pants missing about four feet away.

23 posted on 08/04/2019 11:21:33 AM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv; All

Was the death of this wolf from the meteor strike that destroyed Clovis culture in North America. I know that I have read reports of Siberian Mammoths that were flash frozen so they had undigested buttercup flowers in their stomachs. If so this would explain why the RNA could have been preserved. Flash frozen and then buried without ever thawing until now.

Time for the Firestone, et al book?


24 posted on 08/04/2019 2:56:08 PM PDT by gleeaikin
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To: riverrunner; PIF; SunkenCiv; blam; All

I had a scary experience with a bear in an upper New York state park. My husband, 2 young children, and another couple were camping by a lake. Our food prep was on a picnic table about 25 feet away and we were by the table where we were going to eat. Suddenly we saw a black bear had grabbed a grocery bag from the other table and was starting to run off with it. My husband picked up a 4’ club (actually a small stripped down pine tree) and started after the bear hollering, “Hey, that’s MY food.” The other guy picked up a slightly smaller club and followed him. His wife grabbed the two children and headed off in the other direction. I ran to the car and pulled my 357 pistol out of hiding and ran toward the men and the bear. All the time I was thinking, how do I shoot the bear if it has one of the men down? I decided, run up alongside and shoot in his ear. Fortunately, the bear dropped the bag. We went to sleep well armed in our tents, no food inside. About 6 in the morning we were awakened by yelling. Someone had camped during the night next door. The bear had come and ripped open the shelter over their picnic table with food on it. We rolled over and went back to sleep. When we got up, those people were gone. The next day the ranger said he was just a Yogi type personality bear.


25 posted on 08/04/2019 3:06:59 PM PDT by gleeaikin
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To: null and void; SunkenCiv; All

Have they identified it as a Dire Wolf?


26 posted on 08/04/2019 3:07:53 PM PDT by gleeaikin
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To: gleeaikin

All the time I was thinking, how do I shoot the bear if it has one of the men down? I decided, run up alongside and shoot in his ear.

That is how it is done.

I hunt bears with hounds more then once I have moved in on a bear that had a hound down or was wounded with the pack around it.

I get in close put the muzzle next to his head and drive a bullet into the brain.

Dead bear.


27 posted on 08/04/2019 3:16:32 PM PDT by riverrunner ( o the public,)
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To: SunkenCiv

LOL


28 posted on 08/04/2019 3:49:56 PM PDT by Salamander (Death makes angels of us all, and give us wings where we once had shoulders, smooth as ravens' claws)
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To: gleeaikin
Ooh! Good call!

The Cycle of Cosmic Catastrophes: Flood, Fire, and Famine in the History of Civilization
The Cycle of Cosmic Catastrophes:
Flood, Fire, and Famine
in the History of Civilization

by Richard Firestone,
Allen West, and
Simon Warwick-Smith


29 posted on 08/04/2019 3:55:30 PM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: Salamander

How about wooly rhinos?


30 posted on 08/04/2019 3:59:47 PM PDT by Reily
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To: Reily
I don't like 'em, they tend to horn in. /rimshot

31 posted on 08/04/2019 4:01:01 PM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: 75thOVI; Abathar; agrace; aimhigh; Alice in Wonderland; AnalogReigns; AndrewC; aragorn; ...
Thanks gleeaikin for the suggestion.

32 posted on 08/04/2019 4:01:17 PM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: SunkenCiv

And giant ground sloths are well... go on Pleistocene welfare … hang around, watch the telly, read the racing forms and are just sloths. Big hairy Winslows!


33 posted on 08/04/2019 4:06:27 PM PDT by Reily
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To: Reily
I think I went to school with Harry.

34 posted on 08/04/2019 4:25:01 PM PDT by SunkenCiv (Imagine an imaginary menagerie manager imagining managing an imaginary menagerie.)
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To: gleeaikin

No, I was just making a funny.

I do that.

Sometimes better than others...


35 posted on 08/04/2019 4:57:48 PM PDT by null and void (When the only tool you have is a hammer, ALL your problems look like skulls.)
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To: Reily

Meh.

:)


36 posted on 08/05/2019 12:21:50 AM PDT by Salamander (Death makes angels of us all, and give us wings where we once had shoulders, smooth as ravens' claws)
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To: Reily

Winslow should be Onslow

Onslow = Winslow

From old BBC comedy “Keeping Up Appearances”!


37 posted on 08/05/2019 5:35:24 AM PDT by Reily
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To: null and void

Ha! Nicely done.


38 posted on 08/05/2019 6:32:39 AM PDT by Rebelbase
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To: SunkenCiv

Pun central going on here this morning.


39 posted on 08/05/2019 6:33:25 AM PDT by Rebelbase
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To: Rebelbase
Unbearable...
40 posted on 08/05/2019 6:43:33 AM PDT by null and void (When the only tool you have is a hammer, ALL your problems look like skulls.)
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