"We will collect viral load data from fresh fecal pellets. SARSr-CoV spike proteins will be sequenced, viral recombination events identified, and isolates used to deny strains that can replicate in human cells. The Univ. N Carolina (UNC) team will reverse engineer spike proteins of a large sample of high and low-risk viruses for further characterization. This will effectively freeze the QS we analyze at t=0. These QS0 strain viral spike glycoproteins will be synthesized and those binding to human cell receptor ACE2 will be inserted into SARSr-CoV backbones (non DURC, non-GoF), and inoculated into humanized mice to assess capacity o cause SARS like disease, efficay of monoclonal therapies, the inhibitor G-57348 or vaccines against SARS-Cov8-12. "
https://s3.documentcloud.org/documents/21066966/defuse-proposal.pdf
Yep. Wonder if there is a paper, abstract, or presentation of the results. Will look later.
grey_whiskers wrote:
“
A grant proposal from Daszak in 2018.
“We will collect viral load data from fresh fecal pellets. SARSr-CoV spike proteins will be sequenced, viral recombination events identified, and isolates used to deny strains that can replicate in human cells. The Univ. N Carolina (UNC) team will reverse engineer spike proteins of a large sample of high and low-risk viruses for further characterization. This will effectively freeze the QS we analyze at t=0. These QS0 strain viral spike glycoproteins will be synthesized and those binding to human cell receptor ACE2 will be inserted into SARSr-CoV backbones (non DURC, non-GoF), and inoculated into humanized mice to assess capacity o cause SARS like disease, efficay of monoclonal therapies, the inhibitor G-57348 or vaccines against SARS-Cov8-12. “
https://s3.documentcloud.org/documents/21066966/defuse-proposal.pdf
“
Where did that research go, after UNC?
Outsourced to Wuhan when told they couldn’t do gain-of-function research in the US?
These people need to be strung up for sure.