Coronavirus Live Thread. No. 11. (Weekend)

3/6/2020

[Vermont Lt]

Continuation of Thread

No 10 here:

http://www.freerepublic.com/focus/f-chat/3821921/posts

[Mozilla]

Some videos on the coronavirus for further information.

Coronavirus Pandemic Explained:
https://youtu.be/sWjYs7aILZ8

What is the Coronavirus?:
https://youtu.be/qJKmLTDLyws

What happens if you get Coronavirus?:
https://youtu.be/OTYfke545vI

What is a Pandemic?:
https://youtu.be/oqtfqVsFaqc

Where do new viruses come from?:
https://youtu.be/NJLXdsO1GBI

Coronavirus Epidemic Update:
https://youtu.be/Eeh054-Hx1U

How Coronavirus effects the body:
https://youtu.be/Xj1nUFFVK1E

[BobL]

“...right thru Beverly Hills”

There’s always hope...

[blueplum]

Precursors are simply a recipe of raw materials, the world supply of which China does not control. China manufactures these items because it was an easy task to pass off. Watch how fast we bake the cake if we need to.

Everything China does, can be replicated. Down to rare earth metals - which we have an abundance of. Mr BP works all over the US on very specialized manufacturing equipment. America can produce anything, anywhere, given some lead time. Think WW2 production levels ramped up again. Rising to challenges is in our blood.

[cgbg]

Marathon tomorrow.

Quarantine in a month.

We apes are so clever!

[Em and Brets Mum]

Thank you for Coronavirus Live Thread No. 11 (Weekend). We really appreciate it!

[rodguy911]

Im not really up on the science here maybe someone here can tell me if this is relevant or not: https://www.nature.com/articles/s41422-020-0282-0.pdf LETTER TO THE EDITOR OPEN Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro Cell Research (2020) 30:269–271; https://doi.org/10.1038/s41422-020-0282-0 Dear Editor, In December 2019, a novel pneumonia caused by a previously unknown pathogen emerged in Wuhan, a city of 11 million people in central China. The initial cases were linked to exposures in a seafood market in Wuhan.1 As of January 27, 2020, the Chinese authorities reported 2835 confirmed cases in mainland China, including 81 deaths. Additionally, 19 confirmed cases were identified in Hong Kong, Macao and Taiwan, and 39 imported cases were identified in Thailand, Japan, South Korea, United States, Vietnam, Singapore, Nepal, France, Australia and Canada. The pathogen was soon identified as a novel coronavirus (2019-nCoV), which is closely related to sever acute respiratory syndrome CoV (SARS-CoV).2 Currently, there is no specific treatment against the new virus. Therefore, identifying effective antiviral agents to combat the disease is urgently needed. An efficient approach to drug discovery is to test whether the existing antiviral drugs are effective in treating related viral infections. The 2019-nCoV belongs to Betacoronavirus which also contains SARS-CoV and Middle East respiratory syndrome CoV (MERS-CoV). Several drugs, such as ribavirin, interferon, lopinavir-ritonavir, corticosteroids, have been used in patients with SARS or MERS, although the efficacy of some drugs remains controversial.3 In this study, we evaluated the antiviral efficiency of five FAD-approved drugs including ribavirin, penciclovir, nitazoxanide, nafamostat, chloroquine and two well-known broad-spectrum antiviral drugs remdesivir (GS5734) and favipiravir (T-705) against a clinical isolate of 2019- nCoV in vitro. Standard assays were carried out to measure the effects of these compounds on the cytotoxicity, virus yield and infection rates of 2019-nCoVs. Firstly, the cytotoxicity of the candidate compounds in Vero E6 cells (ATCC-1586) was determined by the CCK8 assay. Then, Vero E6 cells were infected with nCoV2019BetaCoV/Wuhan/WIV04/20192 at a multiplicity of infection (MOI) of 0.05 in the presence of varying concentrations of the test drugs. DMSO was used in the controls. Efficacies were evaluated by quantification of viral copy numbers in the cell supernatant via quantitative real-time RT-PCR (qRT-PCR) and confirmed with visualization of virus nucleoprotein (NP) expression through immunofluorescence microscopy at 48 h post infection (p.i.) (cytopathic effect was not obvious at this time point of infection). Among the seven tested drugs, high concentrations of three nucleoside analogs including ribavirin (half-maximal effective concentration (EC50) = 109.50 μM, halfcytotoxic concentration (CC50) > 400 μM, selectivity index (SI) > 3.65), penciclovir (EC50 = 95.96 μM, CC50 > 400 μM, SI > 4.17) and favipiravir (EC50 = 61.88 μM, CC50 > 400 μM, SI > 6.46) were required to reduce the viral infection (Fig. 1a and Supplementary information, Fig. S1). However, favipiravir has been shown to be 100% effective in protecting mice against Ebola virus challenge, although its EC50 value in Vero E6 cells was as high as 67 μM,4 suggesting further in vivo studies are recommended to evaluate this antiviral nucleoside. Nafamostat, a potent inhibitor of MERS-CoV, which prevents membrane fusion, was inhibitive against the 2019-nCoV infection (EC50 = 22.50 μM, CC50 > 100 μM, SI > 4.44). Nitazoxanide, a commercial antiprotozoal agent with an antiviral potential against a broad range of viruses including human and animal coronaviruses, inhibited the 2019-nCoV at a low-micromolar concentration (EC50 = 2.12 μM; CC50 > 35.53 μM; SI > 16.76). Further in vivo evaluation of this drug against 2019-nCoV infection is recommended. Notably, two compounds remdesivir (EC50 = 0.77 μM; CC50 > 100 μM; SI > 129.87) and chloroquine (EC50 = 1.13 μM; CC50 > 100 μM, SI > 88.50) potently blocked virus infection at low-micromolar concentration and showed high SI (Fig. 1a, b). Remdesivir has been recently recognized as a promising antiviral drug against a wide array of RNA viruses (including SARS/MERS-CoV5 ) infection in cultured cells, mice and nonhuman primate (NHP) models. It is currently under clinical development for the treatment of Ebola virus infection.6 Remdesivir is an adenosine analogue, which incorporates into nascent viral RNA chains and results in pre-mature termination.7 Our time-ofaddition assay showed remdesivir functioned at a stage post virus entry (Fig. 1c, d), which is in agreement with its putative antiviral mechanism as a nucleotide analogue. Warren et al. showed that in NHP model, intravenous administration of 10 mg/kg dose of remdesivir resulted in concomitant persistent levels of its active form in the blood (10 μM) and conferred 100% protection against Ebola virus infection.7 Our data showed that EC90 value of remdesivir against 2019-nCoV in Vero E6 cells was 1.76 μM, suggesting its working concentration is likely to be achieved in NHP. Our preliminary data (Supplementary information, Fig. S2) showed that remdesivir also inhibited virus infection efficiently in a human cell line (human liver cancer Huh-7 cells), which is sensitive to 2019-nCoV.2 Chloroquine, a widely-used anti-malarial and autoimmune disease drug, has recently been reported as a potential broadspectrum antiviral drug.8,9 Chloroquine is known to block virus infection by increasing endosomal pH required for virus/ cell fusion, as well as interfering with the glycosylation of cellular receptors of SARS-CoV.10 Our time-of-addition assay demon

[Black Agnes]

““Pharmaceuticals across the board really are at risk,” said Thomas Bollyky, director of the Global Health Program at the Council on Foreign Relations in Washington, D.C.

“China produces 80 per cent of the world’s supply of active pharmaceutical ingredient. Those are the parts of the pills that actually do something.””

80% sounds like a monopoly to me.

(from the article I linked).

And yes, we can make those, EVENTUALLY.

We don’t even have the equipment to MAKE the equipment to do so right now. That was literally prised off factory floors, loaded on flatbed railcars and shipped to China over the past 25 years.

The last penicillin fermenter in the States closed in ‘04. There are no 30 somethings that have any clue how to do anything like that as they were only in high school when that happened. That knowledge base is 15 years in the past for the 50 somethings who were 30 somethings when that place still operated.

It will take a Manhattan Project to make all this happen.

Meanwhile, quarantines, pandemics and shortages of all the OTHER stuff we need to manufacture that equipment will prevail.

This isn’t doomism. It’s realism. People have to understand we can’t wave a magic wand and have antibiotics produced in this country by Summer. Not in the quantities needed.

[mrsmith]

May help with a point you frequently try to make.
Linked in reply 1193.

[Black Agnes]

Aie, wall of text.

Yes, if you had been on these threads for a month you would have seen that particular drug mentioned many times.

The question is availability and how much of it do we have.

And how do we make more since the precursors to make it are likely in China and they’re not being all giving and generous with that stuff just now.

[BenLurkin]

Murrieta [CA] high school closed, 71 students self-quarantined while staff member is tested for coronavirus

https://ktla.com/news/local-news/71-murrieta-high-school-students-told-to-self-quarantine-while-staff-member-is-tested-for-coronavirus/

[metmom]

Stocking up is the responsible thing to do. It means that your family will not be dependent on government services.

And that’s the problem for the government.

They WANT you dependent on them.

All the more reason to not be.

[Oorang]

#Egypt's health ministry confirms 33 new cases of #coronavirus on Nile cruise ship.

https://twitter.com/i/web/status/1236365962871484422

[rodguy911]

sorry i just ran across it.

[Black Agnes]

It’s OK.

Here’s a good one:

https://www.nejm.org/doi/full/10.1056/NEJMoa2001191

First patient was healthy non smoking 35yr old. Who likely would have suffered severe long term harm to his physical health (lung damage and possibly more) or have been dead.

literally cured overnight with one dose of that drug.

[Raebie]

Would be nice if they applied that WW2 mentality to the test kits. Based on that cluster, I’m not overly optimistic.

[ScottfromNJ]

Steve Lookner
@lookner
·
7m
70 employees of the Washington nursing home are now showing symptoms

[BobL]

“#Egypt’s health ministry confirms 33 new cases of #coronavirus on Nile cruise ship.”

The Houston breakout was from that ship, and the ONLY REASON they even tested the sick people from that ship was because Taiwan had already confirmed that there was cases on that ship, and from the same group of people. Otherwise they would not have met the CDC requirements for testing.

So, had some of them NOT gone home to Taiwan (and been tested), the people in Houston would still be all happy-talk...even today.

[Oorang]

PIERCE COUNTY, Wash. — The first known case of coronavirus has been diagnosed in Pierce County.

Friday evening, officials with the Tacoma-Pierce County Health Department confirmed the patient is a Gig Harbor man in his 50s undergoing treatment at St. Anthony’s Hospital. He first reported symptoms similar to those related to COVID-19 to his personal physician before his condition worsened. He remains hospitalized and is now recovering, according to health department director Dr. Anthony Chen.

It’s not known how the man, whose identity has not been made public, contracted the virus but health department officials say he has not traveled internationally and has had no known contact with anyone who has traveled to a country where coronavirus outbreaks have occurred. Health department officials are interviewing the man to determine who he has made contact with over the past several weeks.

Excerpted

https://www.kiro7.com/news/local/no-cases-coronavirus-found-pierce-county-so-far/7RUSKWXPHFDULE2YAIA2FSJ4H4/

[abigkahuna]

G

[cgbg]

I think that is the nursing home where they stopped testing because CDC said no further information was needed.

You can’t make up this stuff!