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An mRNA COVID vaccine (and potentially more) with nanoparticles, no shot needed
Yale School of Engineering and Applied Science ^ | 08/16/2023

Posted on 10/03/2023 6:47:30 PM PDT by EinNYC

A team of researchers has developed an inhalable vaccine that successfully protects against the COVID virus. It also opens the door to delivering other messenger RNA (mRNA) therapeutics for gene replacement therapy and other treatments in the lungs.

Results of the study, led by Prof. Mark Saltzman, are published in Science Translational Medicine.

For the vaccine, the researchers demonstrated that two intranasal doses of the treatment, made with nanoparticles carrying mRNA COVID vaccine, is effective in mice. It also demonstrates that an inhalable delivery system allows for minimally invasive and lung-targeted mRNA delivery, potentially applicable for numerous pulmonary diseases in addition to COVID.

It’s a significant advance since scientists have had trouble creating lung-targeted mRNA therapies. Typically, these therapies have had poor transfection efficiency - that is, only a small fraction of administered nucleic acids make it into cells that lead to expression of the encoded protein. Also, in the past, the nanoparticles that deliver the mRNA have caused inflammation and other problems. The Saltzman group got around this hurdle in part by using a nanoparticle made from poly(amine-co-ester) polyplexes, or PACE, a biocompatible and highly customizable polymer.

Saltzman previously worked with the lab of Akiko Iwasaki, Sterling Professor of Immunology, on what Iwasaki calls a “prime and spike” COVID vaccine delivery system. The “prime” half of the system involves injections of the mRNA vaccine into a muscle - the shot that millions of people have already received. These vaccinations were followed up with familiar spike proteins or spike mRNA that are derived from the coronavirus and are sprayed directly into the nose.

In their new study, the researchers showed that the shot isn’t necessary to provide protection.

“In the new report, there is no intramuscular injection,” said Saltzman, the Goizueta Foundation Professor of Biomedical Engineering, Chemical & Environmental Engineering & Physiology, and a member of Yale Cancer Center. “We just gave two doses, a prime and a boost, intranasally, and we got a highly protective immune response. But we also showed that, generally, you can deliver different kinds of mRNA. So it's not just good for a vaccine, but potentially also good for gene replacement therapy in diseases like cystic fibrosis and gene editing. We used a vaccine example to show that it works, but it opens the door to doing all these other kinds of interventions.”

Without the protective casing of the nanoparticles, the mRNA would quickly deteriorate inside the body. However, developing a nanoparticle for lung-targeted therapies has been tricky. Other attempts to develop an inhalable delivery system for mRNA have met obstacles due to the type of material used for the nanoparticles.

“It's been a challenge trying to take the lipid nanoparticle vaccine delivery systems and make them active through the nose as well,” Saltzman said. “One of the advantages we have is that the PACE polymer that we're using seems to be much milder, and much better tolerated in the lung than lipid nanoparticles are.”

A previous study from the Saltzman lab characterized PACE polymers with various chemical end-groups.

“There are a lot of chemistries that govern the transfection efficiency of mRNA, and we chose the top candidates and then tested them for our work,” said Hee Won Suh, an associate research scientist in the Saltzman lab, and co-corresponding author of the paper.

It’s a process that took a fair amount of trial and error.

“We have guiding design principles, but it was not perfectly understood which formulation would be best,” said Alexandra Suberi, a PhD student in the Saltzman lab and lead author of the paper. “We screened formulations with varying amine structures and polyethylene glycol content to find a formulation that would work well in lung cells. The end-group and PEG content both had a large effect on protein expression.”

The next step, the researchers say, is to test the delivery system for other therapeutic applications.


TOPICS: Health/Medicine
KEYWORDS: covaxhorror; covid; covid1984; intranasal; mrna; wearedoomed
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To: ransomnote

Pingaling.


21 posted on 10/03/2023 8:23:01 PM PDT by Jane Long (What we were told was a conspiracy theory in ‘20 is now fact. Land of the sheep, home of the knaves)
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To: roving

You don’t know what you are talking about.

The COVID-19 vaccines do not contain any aborted fetal cells. However, fetal cell lines – cells grown in a laboratory based on aborted fetal cells collected generations ago – were used in testing during research and development of the mRNA vaccines, and during production of the Johnson & Johnson vaccine.

The mRNA technology is now in clinical trials for cures to cancer and other diseases. For an informative look into that technology, its history and future, go here:

https://www.pennmedicine.org/mrna


22 posted on 10/03/2023 9:13:57 PM PDT by jonrick46 (Leftniks chase illusions of motherships at the end of the pier.)
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To: SecAmndmt; datura; Fractal Trader; grey_whiskers; metmom; Jane Long; tatown; Golden Eagle; ...

PING to Covid ‘vaccine’

The CDC/FDA and other medical regime players are treating us like we are livestock. No permission sought or required. When you take the cat to the vet, the vet does not consult with the cat as to which vaccines it wants....


23 posted on 10/03/2023 10:45:28 PM PDT by ransomnote (IN GOD WE TRUST)
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To: ransomnote

Our new analysis of @CDCMMWR 's 77 studies pertaining to masks was accepted & published w/no edits requested & it's a scathing commentary on the @CDCgov's flagship "scientific" journal

>75% of reports concluded masks "work" yet only 30% studied masks & 0% found causal evidence… pic.twitter.com/WkINaDM8FP— Tracy Høeg, MD, PhD (@TracyBethHoeg) October 4, 2023


24 posted on 10/03/2023 11:08:31 PM PDT by grey_whiskers ( The opinions are solely those of the author and are subject to change without notice.)
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To: EinNYC

how do you insure they don’t release it in a public area thereby infecting everyone inhaling it with the mRNA virus without any ability to prevent it?


25 posted on 10/03/2023 11:14:59 PM PDT by sten (fighting tyranny never goes out of style)
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To: SoConPubbie
"This is called poisoning boys and girls!"

And crimes against humanity.

26 posted on 10/03/2023 11:25:28 PM PDT by Pajamajan (Pray for our nation. Never be slave in a new Socialist America )
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To: sten
how do you insure they don’t release it in a public area thereby infecting everyone inhaling it with the mRNA virus without any ability to prevent it?

That is my point! This takes the control of getting whatever the government demands you be treated with out of your hands and into theirs.

27 posted on 10/04/2023 8:12:03 AM PDT by EinNYC
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To: LegendHasIt

but potentially also good for gene replacement therapy in diseases like cystic fibrosis and gene editing.

Right out in front of God and everybody else, they are admitting the goal of the shots was altering the human code. I would rather die than allow “them” to mess with my very essence.


28 posted on 10/04/2023 9:16:59 AM PDT by Glad2bnuts (“And how we burned in the camps later, thinking: We should have set up ambushes...paraphrased)
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To: EinNYC

Absolutely not. Spike proteins and nanoparticles right to the lungs? Might as well wrap my lips around the tailpipe of my car and suck it in.


29 posted on 10/04/2023 9:19:33 AM PDT by dforest
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To: dforest
Absolutely not. Spike proteins and nanoparticles right to the lungs? Might as well wrap my lips around the tailpipe of my car and suck it in."

Have you read the article? They have devised a way to insert substances into an aerosol mist which can be breathed in and thus get into the body's circulation. It doesn't require your permission, either. Just you breathing in the air around you.

30 posted on 10/04/2023 9:26:23 AM PDT by EinNYC
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To: EinNYC

Honestly, I believe they are already doing those things behind our backs.

We usually start to hear about what they can do after they have already been doing it.

How do we stop them?


31 posted on 10/04/2023 9:41:33 AM PDT by dforest
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To: EinNYC

32 posted on 10/04/2023 9:45:28 AM PDT by Sirius Lee (They intend to murder us. Prep if you want to live and live like you are prepping for eternal life)
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