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ABSTRACT: Quantitative proteomics reveals a broad‐spectrum antiviral property of Ivermectin, benefiting for COVID‐19 treatment
PMC, US National Library of Medicine, National Institute of Health ^ | 09/20/2020 | Na Li, Lingfeng Zhao, and Xianquan Zhan

Posted on 12/02/2021 10:28:20 PM PST by SeekAndFind

Abstract

Viruses such as human cytomegalovirus (HCMV), human papillomavirus (HPV), Epstein–Barr virus (EBV), human immunodeficiency virus (HIV), and coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS‐CoV‐2]) represent a great burden to human health worldwide.

FDA‐approved anti‐parasite drug ivermectin is also an antibacterial, antiviral, and anticancer agent, which offers more potentiality to improve global public health, and it can effectively inhibit the replication of SARS‐CoV‐2 in vitro.

This study sought to identify ivermectin‐related virus infection pathway alterations in human ovarian cancer cells. Stable isotope labeling by amino acids in cell culture (SILAC) quantitative proteomics was used to analyze human ovarian cancer cells TOV‐21G treated with and without ivermectin (20 μmol/L) for 24 h, which identified 4447 ivermectin‐related proteins in ovarian cancer cells. Pathway network analysis revealed four statistically significant antiviral pathways, including HCMV, HPV, EBV, and HIV1 infection pathways.

Interestingly, compared with the reported 284 SARS‐CoV‐2/COVID‐19‐related genes from GencLip3, we identified 52 SARS‐CoV‐2/COVID‐19‐related protein alterations when treated with and without ivermectin. Protein–protein network (PPI) was constructed based on the interactions between 284 SARS‐CoV‐2/COVID‐19‐related genes and between 52 SARS‐CoV‐2/COVID‐19‐related proteins regulated by ivermectin.

Molecular complex detection analysis of PPI network identified three hub modules, including cytokines and growth factor family, MAP kinase and G‐protein family, and HLA class proteins. Gene Ontology analysis revealed 10 statistically significant cellular components, 13 molecular functions, and 11 biological processes. These findings demonstrate the broad‐spectrum antiviral property of ivermectin benefiting for COVID‐19 treatment in the context of predictive, preventive, and personalized medicine in virus‐related diseases.

1. This study sought to identify ivermectin‐related virus infection pathway alterations in human cells.

2. Quantitative proteomics revealed that ivermectin‐related proteins are involved in four statistically significant antiviral pathways, including human cytomegalovirus (HCMV), human papillomavirus (HPV), Epstein–Barr virus (EBV), human immunodeficiency virus 1 (HIV1), and COVID‐19 infection pathways.

3. We identified 52 severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)/COVID‐19‐related protein alterations when treated with and without ivermectin, and these proteins were involved in cytokines and growth factor family, MAP kinase and G‐protein family, and HLA class proteins.

4. These findings demonstrate the broad‐spectrum antiviral property of ivermectin benefiting for COVID‐19 treatment in the context of predictive, preventive, and personalized medicine in virus‐related diseases.

CLICK ON THIS LINK FOR DETAILS OF THE STUDY



TOPICS: Health/Medicine; Science; Society
KEYWORDS: antiviral; covid19; covidinfo; ivermectin; ivermectinstudy; proteomics

1 posted on 12/02/2021 10:28:20 PM PST by SeekAndFind
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To: Mrs. Don-o; tellw; Huskrrrr; Jane Long; Freedom'sWorthIt; Freedom56v2; BDParrish; Phx_RC; cba123; ..

Ping for your interest


2 posted on 12/02/2021 10:28:50 PM PST by SeekAndFind
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To: SeekAndFind
Great paper. I find the Introduction most illuminating...
Drug repurposing and repositioning [of Ivermectin] has been shown to control a completely new range of diseases (Ashour, 2019). For example:

• orbital myiasis, trichinosis, malaria, leishmaniasis, African trypanosomiasis, asthma, epilepsy, neurological disease, antiviral (e.g., human immunodeficiency virus [HIV], dengue, encephalitis; Yang et al., 2020)

• antibacterial (tuberculosis and Buruli ulcer; Csóka et al., 2018)

• anticancer (breast cancer, leukemia, glioblastoma, cervical cancer, gastric cancer, ovarian cancer, colon cancer, melanoma, and lung cancer; Crump, 2017).

Multifaceted ‘wonder’ ivermectin may become an even more exceptional drug in the future. An international patent ‘Use of ivermectin and derivatives thereof’ caught people's increasing attention to ivermectin those years. Ivermectin would be developed to use for metabolic‐related diseases (diabetes, hypercholesterolemia, insulin resistance, obesity, hypertriglyceridemia, and hyperglycemia), Famesoid X receptor‐mediated diseases (atherosclerosis, nonalcohol fatty liver disease, cholestasia, and gallstones), inflammation, and cancer (Crump, 2017).

It is truly astonishing that one drug, derived from nature, could possibly fight all of those diseases.

But NEVER FORGET, it's a Horse Dewormer, nothing more, and a Horse Dewormer it shall remain forever and ever.

3 posted on 12/02/2021 10:45:42 PM PST by ProtectOurFreedom (“…in any great disaster, there's a Harvard man in the middle of it.” ~ Thomas Sowell)
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To: ProtectOurFreedom
Do your homework.

Merck developed Ivemectin in 1980. 3.5 billion doses have been given to humans.

Here’s the history of the drug:

https://rumble.com/vlpecw-the-story-of-ivermectin.html

4 posted on 12/02/2021 10:53:52 PM PST by Cobra64 (Common sense isn’t common anymore.)
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To: Cobra64

“Do your homework.”

WTH? Do I really need to tell you my last line was tongue in cheek and declaring the absolute ridiculous statement by CDC (or was it FDA)?


5 posted on 12/02/2021 10:59:01 PM PST by ProtectOurFreedom (“…in any great disaster, there's a Harvard man in the middle of it.” ~ Thomas Sowell)
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To: SeekAndFind

Just horse paste to the idiotic Left tho, so yeah........


6 posted on 12/02/2021 10:59:27 PM PST by cranked
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To: SeekAndFind

There was a lot of research on the repurposing the drug known as phenytoin( Dilantin) a long time ago. When they find something extra that ivermectin does that is more useful in some medical condition...some pharmaceutical chemist will synthesize a newer agent that is more potent than ivermectin and try and bring it to market....then the politicking and influence peddling, and data manipulation, and lobbying begins...reminiscent about the old observation about not wanting to see how sausage and laws are made...


7 posted on 12/03/2021 12:14:58 AM PST by Getready (Wisdom is more valuable than gold and diamonds, and harder to find.)
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cool - bmfl


8 posted on 12/03/2021 5:44:45 AM PST by Moltke (Reasoning with a liberal is like watering a rock in the hope to grow a building.)
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To: ransomnote

Ping.


9 posted on 12/03/2021 5:57:35 AM PST by upchuck (The longer I remain unjabbed with the clot-shot, the more evidence I see supporting my decision.)
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