During His last Passover meal, Jesus stops at the 3rd cup, passes the bread and wine, makes His " This is my Body " discourse, and then we read that the apostles sang a hymn, and then went outside.. The ritual Passover had 4 cups of wine AND the Lamb is eaten by all. In the Last Supper, the only clear "lamb" that was eaten was Jesus Body and Blood, and the 4th Cup came at his death: " I will not drink of the fruit of the vine until...".
this is why He refused the wine/water offered to Him on the way to Calvary.
The Fourth Cup: The Sacrament of the Eucharist [Holy Thursday] [Passover]