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To: E. Pluribus Unum
Those were deliberately generated false-positives.

No, a false positive cannot be deliberately generated. Since PCR samples are run next to negative and positive controls, if the reagent mixture gets contaminated such that the reaction shows a positive when the target nucleic acid is not present, the negative control will also show a positive result. Once that happens, the entire PCR assay is discarded, the area where the PCR reactions are mixed is cleaned and the assay is repeated with all new tubes and pipettes on clean equipment. The PCR assay would also be repeated if the positive control came up negative.

Like I said, try to learn something about how PCR is conducted.

How does PCR work and what are its 3 steps?

13 posted on 10/07/2025 6:30:10 PM PDT by exDemMom (Dr. exDemMom, infectious disease and vaccines research specialist.)
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To: exDemMom

A recipe for generating false positives when desired. How quaint.


15 posted on 10/07/2025 6:34:19 PM PDT by E. Pluribus Unum (Je suis Charlie Kirk.)
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To: exDemMom

So you have no interest in determining whether a something detected at 40 cycles is actually a health threat, you just want to mindle$$ly crank out numbers and pretend you have discovered a deadly asymptomatic infection?


16 posted on 10/07/2025 6:38:27 PM PDT by E. Pluribus Unum (Je suis Charlie Kirk.)
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