Circulating SARS-CoV-2 Vaccine Antigen Detected in the Plasma of mRNA-1273 Vaccine Recipients

Alex Berenson ^ | May 20, 2021 | Alana F Ogata, Chi-An Cheng, Michaël Desjardins, Yasmeen Senussi, Amy C Sherman, Megan Powell, Lewis

[Cathi]

SARS-CoV-2 proteins were measured in longitudinal plasma samples collected from 13 participants who received two doses of mRNA-1273 vaccine. 11 of 13 participants showed detectable levels of SARS-CoV-2 protein as early as day one after first vaccine injection. Clearance of detectable SARS-CoV-2 protein correlated with production of IgG and IgA.

Here, we provide evidence that circulating SARS-CoV-2 proteins are present in the plasma of participants vaccinated with the mRNA-1273 vaccine. We report antigen and serological data of the mRNA-1273 vaccine in 13 healthcare workers at the Brigham and Women’s Hospital. Ultrasensitive single-molecule array (Simoa) assays were used for the detection of SARS-CoV-2 antigens spike (S1-S2 unit), S1, and nucleocapsid and antibodies IgG, IgA, and IgM against SARS-CoV-2 spike, S1, receptor binding domain (RBD), and nucleocapsid, as previously described6,7. The ultralow detection limits of the Simoa assays enable detection of antigen and antibody production in the early stages post vaccination and quantification of changes in levels over the course of both vaccine injections.

[BDParrish]

Significance?

The Simoa Assays are ultra sensative allowing them to verify that the vaccines began working immediately. They also think they will be able to chart the increases in antibody production, what they call "antibody dynamics, in the subjects each day over the course of two shots. Previous information did not include actual data that the spike protein was in fact being created and they are happy to say they found it, telling you exactly how and who. Here is the money quote:

"However, critical data demonstrating the direct production of spike protein via translation from the mRNA-1273 vaccine in these studies are missing, precluding a full understanding of the vaccine mechanism. Here, we provide evidence that circulating SARS-CoV-2 proteins are present in the plasma of participants vaccinated with the mRNA-1273 vaccine."

I appreciate Cathi and expect her to ferret out these things for us. But this article will not be it. Please correct me with my thanks!

[reformedliberal]

...we were previously reassured that they remained in the cell and did not circulate in the bloodstream.
___________________________

This is confusing to me.

My understanding is that the IM injection in the deltoid (or the gluteals)is precisely _because_ these muscles are richly vascularized.

At some point, I even ran across a *flow* chart from capillary to minor and major veins subsequent to a deltoid IM injection.

How would any injection be of value if it did not enter the blood stream? How could anyone assert that anything injected into a muscle full of capillaries would not 1) diffuse into those capillaries and 2) from there, continue along the venous pathway?

Seems to me, with a basic, if rudimentary, understanding of the human body, that anything injected into a muscle is _intended_ to diffuse into the bloodstream and, if it did not, it would simply pool uncomfortably in the muscle until....??? What else was the injection supposed to do and where else was it supposed to go and how was any route other than eventual blood system expected to accomplish an immunization?

How else does the *vaccine* enter any cell other than those at the injection site if not through the blood vessels???

I have read that once the spike protein encounters the vessel wall or the endothelium of an organ, it sticks there, by design. How else would an immune cell find this spike protein if not via the bloodstream?

Further, if the design of the *operating system* is to turn the injected host into a producer of spike protein, it seems then that the body itself would continue to produce them and it would be inevitable that some spike proteins would be circulating in the blood.

What am I missing in regard to the “reassurance that they remained in the cell”?

[Cathi]

This is from Derek Lowe’s science website: “Now we get to a key difference: when a cell gets the effect of an mRNA nanoparticle or an adenovirus vector, it of course starts to express the Spike protein. But instead of that being assembled into more infectious viral particles, as would happen in a real coronavirus infection, this protein gets moved up to the surface of the cell, where it stays. That’s where it’s presented to the immune system, as an abnormal intruding protein on a cell surface. The Spike protein is not released to wander freely through the bloodstream by itself, because it has a transmembrane anchor region that (as the name implies) leaves it stuck. That’s how it sits in the virus itself, and it does the same in human cells.”

“So the muscle cells around the injection are hit by the vaccine (whether mRNA-containing lipid nanoparticles or adenovirus vectors) while a good portion of the remaining dose is in the intercellular fluid and thus drains through the lymphatic system, not the bloodstream. That’s what you want, since the lymph nodes are a major site of immune response.”

Now I have no idea if his explanation was accurate or not; but that is the theory behind not being alarmed by the recent Salk study that showed that the Spike proteins themselves are a major cause of damage to various organs in covid. The Salk study says that covid is a vascular disease, not a respiratory one and the Spike protein causes the damage in covid in all the various organs. Under no circumstances do scientists want Spike proteins in the bloodstream.

The VAERS reports of adverse events (injuries) in cardiovascular, neurological, brain, skin, blood clots, strokes, etc. suggest that it could be the Spike proteins doing the damage.

So the new Harvard study that just came out that shows Spike protein in the plasma of vaccinated individuals and the theory they suggested “We hypothesize that the cellular immune responses triggered by T-cell activation, which would occur days after the vaccination, lead to direct killing of cells presenting spike protein and an additional release of spike into the blood stream9”

As must be clear by now, scientists are in a discovery phase of mRNA technology and everything seems to change with each new scientific paper.