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To: NachOsten

If you didn’t know...
Charles University in Prague, Univerzita Karlova v Praze
Type Public, Ancient
Established - 1348; 673 years ago

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What does that have to do with anything?


20 posted on 02/07/2021 11:01:16 AM PST by ifinnegan (Democrats kill babies and harvest their organs to sell)
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To: ifinnegan
National Academy of Sciences

Structure of the SARS-CoV-2 RNA-dependent RNA polymerase in the presence of favipiravir-RTP

RNA Synthesis The RNA template, 5′-rUrUrUrUrUrCrArUrArArCrUrUrArArUrCrUrCrAr- CrArUrArGrCrArCrUrG-3′, and RNA primer 5′-rCrArGrUrGrCrUrArUrGrUr- GrArGrArUrUrArArGrUrUrArU-3′ were prepared by solid-phase synthesis on an ÄKTA oligopilot plus 10 (Cytiva). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55 °C for 30 min; then the silyl protecting groups were removed by treating with 3 mL of 1:1 mixture of triethylamine trihydrofluoride and dimethyl sulfoxide at 55 °C for 90 min. Then, 30 mL of cold 50 mM NaClO4 in acetone was added to precipitate the RNA product. After centrifugation, the pellet of RNA was dissolved in 5 mL of water and passed through a Sep-Pack C18 Cartridge, 5 g sorbent (Waters). Eluates containing RNA were combined and lyophilized. Mass spectroscopy of the template RNA found m/z ([M-7H+]) = 1,341.8 (theoretical 1,342.1) and found m/z ([M-6H+]) = 1,565.6 (theoretical 1,565.9); mass spectrometry of the primer RNA found m/z ([M-6H+]) = 1,278.6 (theoretical 1,278.9) and found m/z ([M-5H+]) = 1,534.5 (theoretical 1,534.9).

If you can explain to me that article, I will write to her and ask her to sent me her research work. Thanks

32 posted on 02/07/2021 2:08:02 PM PST by NachOsten (Alea iacta est!)
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To: ifinnegan

I get nothing but things telling me about the Charles University in Prague. So would you be so kind as to provide what you are talking about? Thanks in advance.


37 posted on 02/07/2021 3:01:18 PM PST by Robert DeLong
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