Fun! Here are some of my favorites, though not necessarily in the AFI running:
"I am Connor MacLeod of the Clan MacLeod. I was born in 1518 in the village of Glenfinnan on the shores of Loch Shiel. And I am immortal." - (Connor MacLeod, Highlander, 1986)
"What makes a king out of a slave? Courage! What makes the flag on the mast to wave? Courage! What makes the elephant charge his tusk in the misty mist, or the dusky dusk? What makes the muskrat guard his musk? Courage! What makes the sphinx the seventh wonder? Courage! What makes the dawn come up like thunder? Courage! What makes the Hottentot so hot? What puts the "ape" in apricot? What have they got that I ain't got? All: Courage!" - (Bert Lahr, The Cowardly Lion, Wizard of Oz, 1939)
"The public doesn't give a damn about integrity. A town that won't defend itself deserves no help." - (Lon Chaney Jr as 'Howe' in High Noon, 1952)
""I am Connor MacLeod of the Clan MacLeod. I was born in 1518 in the village of Glenfinnan on the shores of Loch Shiel. And I am immortal." - (Connor MacLeod, Highlander, 1986)"
In a similar vein, from the movie that started the Highlander TV show:
"Aye, we're klansmen!"