[DannyTN]

This doesn't make sense to me... When the mice were deprived of 14-3-3 zeta they demonstrated accelerated disease progression.

• then developed a novel protein-based vaccine to stimulate production of anti-14-3-3 zeta antibodies and it successfully prevented the development of disease in several animal models.

depriving the mice of 14-3-3 increased the arthritis. So the team stimulated antibodies against 14-3-3 and that cured them?

it seems like both are going in the same direction. They should have increased the 14-3-3 zeta not antibodies against it?

[Dave Wright]

Its a little confusing but the original article explains it like this. When they knocked out the gene for the protein first it triggered inflammatory arthritis and also reduced the corresponding antibodies for the protein.

When they next tried to reverse the arthritis they had induced with the vaccine to generate the missing antibodies it didn’t reverse the arthritis.

When they vaccinated the mice first before knocking out the gene that made the protein it did prevent arthritis. The lesson is they must use the vaccine preemptively before symptoms develop in the joints for it to prevent the onset of arthritis. It doesn’t cure arthritis, it just prevents it from occurring if the protein production is disrupted.