I don't know what you mean by cut off line. That's like saying "where's the cut off line where the Big Bang ends and gravity begins?" It's a meaningless question.
Of course not, but I’ll try again.
If evolution only addresses the evolution of life, then it behooves one not only to define life but also to decide where the starting point is.
At what point does the ToE kick in?
I seriously doubt that anyone would not consider any single celled organism to be life. But what about other things where they are not so easily delineated?
What about prions? Viruses?
They exhibit some signs of being living but not all. Where do they fall?