I thought the Australian aborigines had been isolated for something like 40,000 years.
-ccm
However, in the Creationist model, the universe and world are some 6,000 Earth years old. Some 5,000 years ago the global population was wiped out except for one family from which every single human today is descended. Thus, at the very greatest extent, two people could be at most cousins separated by some 5,000 years.