“I’m not bluffing” = “I’m bluffing”.
Kind of like Biden’s “I’m not joking”
Given that this is being said to Biden and given that Biden is dumb as a box of rocks, it is quite possible that Putin is telling Biden he's not bluffing because otherwise Biden just wouldn't get it.
Putin is basically saying to Biden, "now pay attention, Big Guy. You need to realize what I'm saying here."
Putin has some left over from all the nukes he used in 2014. *facepalm*
“I’m not bluffing” = “I’m bluffing”.
As Obama once said to GOP, “Don’t call my bluff”.