[Antonello]

How many base pairs in the target gene?

It depends on the temperature, since G. stearothermophilus RNA denatures with an increase in heat. At 20^oC, A-U is 17+/-2 and G-C is 29+/-2. At 52^oC, A-U is 7+/-2 and G-C is 25+/-2.

What is the mutation rate per generation?

0.4% (1 base in 250 mutated) per generation.

How many bases were impervious to mutation?

8+/-3.

At least one of the above answers is correct and at least one is incorrect. Please identify and correct any wrong answers.

[grey_whiskers]

At 20^oC, A-U is 17+/-2 and G-C is 29+/-2. At 52oC, A-U is 7+/-2 and G-C is 25+/-2.

What are the units on the 17+/-2, 29+/2, etc., please?

Cheers!