Doesn't matter. Money is fungible.
What *does* matter is that Highway Funds aren't counted to offset our federal budget deficit numbers.
That answer says a lot. A US bond is a claim on future tax revenue. If highway funds are held as bonds, then the money was spent, and replaced with a claim against future revenues, which makes it part of the deficit. Accounting bonds as cash is one method of understating the deficit.
Do I gather correctly from your reply that you're a right-leaning statist?