No, and no.
No, the bacteria didn't acquire a new property, and no, mere natural selection (without mutation) is not Evolution.
Consider, if you applied an antibiotic to which no member of a colony was immune, the entire colony would *either* have to die or else instantaneously mutate.
Instantaneous mutation never happens in real life. Instead, some existing members of said bacteria colony will already have a trait that makes them resistant to the applied antibiotic. They will then thrive and multiply (no more competition from their now-dead peers, for instance).
Thus, a colony that isn't wiped out soon returns with a widespread resistance to the original antibiotic.
But that effect is due only to Natural Selection, not instantaneous mutation.
No, and no.Yes, and yes. I've done this myself.
if you applied an antibiotic to which no member of a colony was immune, the entire colony would *either* have to die or else instantaneously mutate.Oh please! "incrementally add the antibiotic to the colony" - you don't drown the colony in antibiotics from start. If one is in a real hurry (as you seem to be) add a mutagen.
You seem to have trouble reading. The size of the original colony is one individual.