THREE METHODS FOR GENERATING HUMANIZED MICE
https://www.taconic.com/taconic-insights/oncology-immuno-oncology/methods-generating-humanized-mice.html#:~:text=There%20are%20three%20ways%20to%20humanize%20a%20mouse%3A,human%20donor%20into%20a%20germ-free%20mouse%201%2C%202.
That does not answer my question. I want to know what method Regeneron used for their humanized mice used in their antibody treatment.
This how they did it in 2017:
“Fetal liver samples were obtained from the Human Fetal Tissue Repository at Albert Einstein College of Medicine, Bronx, NY, and from Advanced Biosciences Resources, Alameda, CA. Tissues were cut in small fragments, treated for 45 minutes at 37°C with Collagenase D (100 ng/mL; Roche) and a cell suspension was prepared. Human CD34+ cells were purified by density gradient centrifugation, followed by positive immunomagnetic selection using anti-human CD34+ microbeads (Miltenyi Biotec). Newborn pups were sublethally irradiated with 180 cGy and 100 000 CD34+ cells were injected into the liver. The mice were bled 8 to 10 weeks post-engraftment to check for the reconstitution of the human immune system. More than 20 fetal liver samples were used and the same donor sample was transplanted for both human IL-6–positive and negative recipients to exclude variations that related to the donor.”
In the acknowledgments it reads “The authors thank the Regeneron Pharmaceuticals’ Velocigene team for generating human IL-6 knock-in mice”
https://www.sciencedirect.com/science/article/pii/S0006497120336673#ceack10
I want to know if this how they humanized their mice for regen-cov2.