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To: SeekAndFind

HOW THE NOVAVAX VACCINE WORKS

Novavax's coronavirus vaccine, called NVX-CoV2373, differs from the three vaccines already being distributed in the United States. The vaccine relies on what is called recombinant nanoparticle technology and Novavax's adjuvant, called Matrix-M, to stimulate an immune response and stimulate high levels of neutralizing antibodies.

When the genetic sequence for the coronavirus was published, Novavax scientists identified the gene for the spike protein and created a modified version of that gene. The researchers cloned the genes into a baculovirus that infects insects. They then infected moth cells -- specifically, cells from the fall armyworm insect -- with that virus, prompting them to produce the coronavirus spike protein.

These virus-like nanoparticles were harvested to make Novavax's vaccine.

"The whole idea of the vaccine is to show the immune system something that looks, tastes and acts like a virus, with the exception that it doesn't make you sick. So we made the spike protein. We put it in a particle -- basically, like a soap bubble -- and it's the size of the virus," Glenn said.

"It's not infectious. We never touch the coronavirus itself," he added. "Then that is given to people, and they make an immune response that's very much focused just on the spike -- and I would say, the hallmark of our vaccine is it gives a very strong immune response with very few side effects, and the dose is very small and the vaccine can be stored with normal refrigerated temperatures."

That's different from the Covid-19 vaccines made using messenger RNA carried in fatty particles called lipids. They're more fragile and must be kept frozen.
2 posted on 04/04/2021 12:23:14 PM PDT by SeekAndFind
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To: SeekAndFind

THE FOUR MAIN TYPES OF CORONAVIRUS VACCINES IN DEVELOPMENT

mRNA VACCINE

Used by: Pfizer, Moderna
Doses: 2
mRNA vaccines are the newest approach. They use genetic material called messenger RNA, a kind of genetic software that instructs cells to make a piece of the coronavirus spike protein. That will get the attention of the immune system. The mRNA is coated in soft fatty lipids to protect it.

VECTOR VACCINES

Used by: AstraZeneca, Janssen, Sputnik
Doses: 1-2

Vector vaccines use another virus to carry in the genetic instructions to make the spike protein. For coronavirus they all use adenoviruses, a type of common cold virus. They attach to cells and inject DNA that tells the cells to make coronavirus spike protein.

PROTEIN SUBUNIT VACCINE

Used by: Novavax, Sanofi
Doses: 1-2

Protein subunit vaccines just get little pieces of the target virus circulating in the system for the immune system to find and recognize. Instead of using the human body as the vaccine factory, genetically engineered insect viruses are used to infect moths, whose cells then produce the pieces of coronavirus spike protein. These are harvested and made into a vaccine.

WHOLE, KILLED VACCINES

Used by: Sinovac
Doses: 1

Whole inactivated virus vaccines take longer to make because batches of the coronavirus must first be grown and then killed using a chemical or heat, and then made into a vaccine that can be injected to elicit an immune reponse.
3 posted on 04/04/2021 12:31:02 PM PDT by SeekAndFind
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