Posted on 05/02/2020 8:24:19 PM PDT by Olog-hai
The Chinese are smart. They know that if you delete something from the internet, it just goes away.
The Chinese don’t just delete things.
They derete things. Much more effective.
Once again a UK paper leads the way.
But I really can’t fault American papers for not breaking this story. After all, they are very busy trying to see if Trump got a parking ticket while he was in college.
They intended to spread it.
A guy orders chinese for delivery.
The delivery guy drives up and the homeowner goes outside.
The delivery guy sees the homeowner walking towards him.
And shouts isolate isolate.
The homeowner says, no you’re not, I only ordered it 15min ago.
Chy-na will wear
the pandemic symbol
forever.
There’s no pandemic without “Pand(a)”.
The Chicoms are the paper tiger and house of cards that America seems to be acting like. Get a f-ing set America!!!!
These evil bastards delete people
What could possibly go wrong?
What could possibly go wrong?
______________________________________________________
Wild-type SARS-CoV (Urbani), mouse-adapted SARS-CoV (MA15) and chimeric SARS-like CoVs were cultured on Vero E6 cells (obtained from United States Army Medical Research Institute of Infectious Diseases), grown in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, CA) and 5% fetal clone serum (FCS) (Hyclone, South Logan, UT) along with antibiotic/antimycotic (Gibco, Carlsbad, CA). DBT cells (Baric laboratory, source unknown) expressing ACE2 orthologs have been previously described for both human and civet; bat Ace2 sequence was based on that from Rhinolophus leschenaulti, and DBT cells expressing bat Ace2 were established as described previously8. Pseudotyping experiments were similar to those using an HIV-based pseudovirus, prepared as previously described10, and examined on HeLa cells (Wuhan Institute of Virology) that expressed ACE2 orthologs.
Construction of SARS-like chimeric viruses.
Both wild-type and chimeric viruses were derived from either SARS-CoV Urbani or the corresponding mouse-adapted (SARS-CoV MA15) infectious clone (ic) as previously described27. Plasmids containing spike sequences for SHC014 were extracted by restriction digest and ligated into the E and F plasmid of the MA15 infectious clone. The clone was designed and purchased from Bio Basic as six contiguous cDNAs using published sequences flanked by unique class II restriction endonuclease sites (BglI). Thereafter, plasmids containing wild-type, chimeric SARS-CoV and SHC014-CoV genome fragments were amplified, excised, ligated and purified. In vitro transcription reactions were then preformed to synthesize full-length genomic RNA, which was transfected into Vero E6 cells as previously described2. The medium from transfected cells was harvested and served as seed stocks for subsequent experiments. Chimeric and full-length viruses were confirmed by sequence analysis before use in these studies. Synthetic construction of chimeric mutant and full-length SHC014-CoV was approved by the University of North Carolina Institutional Biosafety Committee and the Dual Use Research of Concern committee.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797993/
All they had to do is wear cloth masks or handkerchiefs to protect themselves from the virus right?
Possibly leaked intelligence report names “patient zero” as Huang Yan Ling, a young lab worker who has since disappeared.
Stop spreading that conspiracy theory. /s
http://www.freerepublic.com/focus/f-bloggers/3834835/posts
they are easing us into a second excuse, we made a mistake .
They also delete people. They’re frighteningly effective at that.
Remember, if you’re going to be handling deadly BL-4 level rated viruses, *please* don’t forget to wear a condom! — every liberal ever
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