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I’ve heard mountain lions calling many nights and have spotted them with a light when they were making the noise. It’s the only noise that I’ve ever heard them make. They make short screeching noises, like some kind of bird. They’d usually make that noise for quite a while. I’ve heard it for up to an hour or so, sometimes with several cats making it (Colorado Rockies from about 8,500 feet elevation to about 9,500 feet). Most often heard them make that noise very late at night.

I finally found a sound file with the same noise after hearing them make it for several years. The description with the file said that it is a female mating call.