[AndrewC]

A hot spot based upon what?

Based upon the fact that a single base position was mutated at least 3 times.

[allmendream]

Yes and 15 of the 17 codons were mutated. I guess they are all hot spots. The third base of a codon is especially subject to differences between species due to the redundant nature of the genetic code. Many mutations at the third position lead to no difference in the amino acid it codes for or a conservative substitution and are thus neutral.