Yes. All of these programs depend on how fast they can track down and counter the newest spyware and that you keep the databases updated (just like your antivirus). None are perfect but using 2 or 3 of the best can keep you very clean. I also use Ad-Aware but somebody else had already mentioned it.
Anyway, if you use more than one, you can see what the others may be missing!
And a Happy Thanksgiving to you all as well.
But Spysweeper found at least four times the amount of spyware on my system then Spybot! I trusted Spybot and it wasn't even a close comparision.
What I did was run Spybot and it found 20 items, did not remove any items. Ran Spysweeper immediately after and it found 84 items. I was a little surprised to say the least.