You never clicked through to the studies that were referenced did you? You dismissed out of hand the leads that were presented by trying to discredit the authors as mere blog authors. Poor form.
You never explained why Pseudouridine was substituted for Uridine and what happens.
https://onlinelibrary.wiley.com/doi/epdf/10.1002/prca.202300048?utm_source=substack&utm_medium=email
Explain this.
“Results: The specific PP-Spike fragment was found in 50% of the biological samples analyzed, and its presence was independent of the SARS-CoV-2 IgG antibody titer. The minimum and maximum time at which PP-Spike was detected after vaccination was 69 and 187 days, respectively.”
187 days was the upper limit of the study not the life of the modified mRNA.
I can recognize instantly the difference between a blog and a link to a legitimate peer-reviewed medical journal. I've been reading medical literature for years. While I can sometimes derive the original medical article that the blog misrepresents, it actually takes time to do this kind of in-depth research. And you had already provided a link to a real medical article that I could use to debunk at least one of the claims that you posted here.
You never explained why Pseudouridine was substituted for Uridine and what happens.
The purpose of substituting pseudouridine for uridine is to bypass the PAMP recognition function of the innate immune system which would normally react to RNA. This substitution does not change the mRNA interactions with the ribosome or alter its translation into protein. Nor does it affect the ability of RNAse to destroy the mRNA after translation. Pseudouridine is naturally produced by your body, so this is not some foreign substance being introduced.
Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines.
mRNA vaccines — a new era in vaccinology. Note that this reference was published in 2018--before anyone ever even imagined something like Covid.
What these two references show is that the mRNA technology was in R&D long before Covid and that optimization of the technology has been a research topic for a while.
Explain this.
“Results: The specific PP-Spike fragment was found in 50% of the biological samples analyzed, and its presence was independent of the SARS-CoV-2 IgG antibody titer. The minimum and maximum time at which PP-Spike was detected after vaccination was 69 and 187 days, respectively.”
Part of the explanation of this is contained in the paper that you linked. The "PP-Spike" fragment is a spike protein in which two adjacent amino acids were replaced by proline in order to stabilize the structure and prevent it from entering the cell's cytosol. Normally, spike protein on the virus surface undergoes processing (cutting and refolding) that causes it to enter the cell cytosol, along with the rest of the virus.
The authors of the paper did not investigate how the PP-spike protein was in the blood. Spike protein is a membrane-bound protein which is unlikely to be free-floating in the blood. They used whole blood samples, which means that it could have been present on the surfaces of blood cells. They described taking blood samples from Covid survivors but did not show the MS-HPLC chromatograms from these patients. They only showed the chromatograms from vaccinated patients and unvaccinated patients who did not have Covid. So I would be curious to see how spike protein quantities compare between vaccinated patients and Covid survivors.
As I mentioned in a previous post, mRNA contained within a nanoliposome is protected from destructive enzymes. There is a property called "associative constant" which tells us how much of two molecules will actually attach to each other at various concentrations. For example, if both molecules are present at 50% concentration, 25% of them might attach to each other, leaving the other 25% unbound. But if both molecules are present at 5% concentration, only 0.1% of them might attach, leaving 4.9% still free-floating. These kinds of calculations are called kinetics. I do not know the specific kinetics of mRNA-containing nanoliposomes to the cells in the lymph nodes, but if it is anything like the kinetics of liposome attachment to cells in culture, it is probably rather inefficient. Those vaccine particles that do not attach immediately will eventually attach, but how long that will take can vary. This would explain continued PP-spike protein production. In theory, this would help to reinforce the immune response since that PP-spike protein remains to keep activating the immune system.
I should point out that actual SARS-CoV-2 virus (containing several viral proteins and mRNAs) can remain in circulation for prolonged periods in people who have had Covid. When you recover from a viral infection, the virus does not always disappear. Herpes viruses, for example, remain for the rest of your life. The persistence of SARS-CoV-2 virus after infection is one of the mechanisms hypothesized for long Covid. This hypothesis is supported by the fact that some people who have long Covid improve after receiving the Covid vaccine. This would be because the vaccine causes their immune system to make spike protein specific antibodies, which then target remaining virus particles for elimination by the immune system.
No doubt, whoever (Robert Malone?) is producing all of this material knows that the people he writes it for don't know how to read the scientific literature and do not have the educational background to interpret it. On the other hand, I try to keep my explanations as accessible as possible to ordinary people who do not have a STEM education.