Turns out the husband had a fraternal twin brother, briefly, while in utero, and when that brother's embryo had spontaneously stopped developing and broken up, parts of it had been incorporated into the husband's embryo, so that his blood and semen did not have identical DNA.
The original C.S.I. show dealt with this in an episode back in 2004. A rapist is identified in a line-up but his DNA swab doesn’t match the rape kit sample. It shows a close male relative, but doesn’t match any of the brothers either.