Sylvia Meagher, Accessories After the Fact: The Warren Commission, The Authorities & The Report, Vintage/Random House 1967/1976, page 120:
Neither Day nor any other police officer seems to have checked the rifle when it was discovered for signs--such as traces of fresh gunpowder inside the barrel--indicating that the weapon had actually been fired that day; FBI Expert Robert Frazier, who examined the rifle early the next morning, when asked if there was metal fouling in the barrel, replied, "I did not examinie it for that." [3H 395]
No such test exists. There are only indicators that show that a gun has been fired.