The Gadsden and other rattlesnake flags were widely used during the American Revolution. There was no standard American flag at the time. People were free to choose their own banners. One of the most interesting variations is the flag of Colonel John Proctor's Independent Battalion from Westmoreland County, Pennsylvania. The flag had an open red field with the British ensign in the upper corner. They painted a coiled rattlesnake and its "Don't Tread on Me" warning onto the center, as if ready to strike at the Union Jack.
The "First Navy Jack" features a rattlesnake winding its way across a field of thirteen red and white stripes.
Southern and Proud! - Katherine Jenerette