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To: Cold Heat

I hope it’s just malaria.

I read in another thread that ebola and malaria can be confused

If a lot of these suspected cases really turn out to be malaria that will be a relief.


68 posted on 10/05/2014 1:17:15 AM PDT by Califreak (Hope and Che'nge is killing U.S.)
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To: Califreak

FYI

“Hardware expenses:
New thermocyclers start at about $2000 and go up. Way up.
Gel running rig including gel caster, combs, powersupply and connectors: $500 and up.
UV transilluminator and image documentation system $7000 and up.
Microwave oven ~$100
Glassware for consumables ~$100

Consumables:
Agarose $200 for 500g
TAE or (TBE) buffer 4 liters of 50x or (10x) concentrate $50
Ethidum Bromide 1 gram $40

Hazardous waste disposal cost: check with chemical waste disposal facility

Primers ~$10 each for 25 nMol scale synthesis
Enzyme and buffers $50 and up for cheap Taq
dNTPs 1000 ul 10mM each dNTP $50
Molecular size markers and loading dye $50

Time to run program with small PCR amplicons: 2-3 hours.

Time to understand all of the above: 4 years of undergraduate work and a couple years of post-graduate training.


71 posted on 10/05/2014 1:21:43 AM PDT by Cold Heat (Have you reached your breaking point yet? If not now....then when?)
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