Here are the articles (the relevant parts) you call “outdated” but, of course, you offer nothing to support your statement:
“Structure of Chicken Hypothalamic Luteinizing Hormone-releasing
Hormone
II. ISOLATION AND CHARACTERIZATION*
(Received for publication, February 17, 1982)
Judy A. King and Robert P. Millar
From the Department of Chemical Pathology, University of Cape Town Medical School and Groote Schuur Hospital,
Observatory 7925, Cape Town, Republic of South Africa
The decapeptide LH-RH’ (pGlu-His-Trp-Ser-Tyr-Gly-Leu-
Arg-Pro-Gly-NH 2 ), originally isolated from porcine hypothalami
(Matsuo et al., 1971a) and later synthesized (Matsuo
et al., 1971b), appears to have lower gonadotropin-releasing
activity in submammalian vertebrates than in mammals.
Crude hypothalamic extracts from birds, reptiles, amphibians,
and fish, however, contain significant gonadotropin-releasing
activity, and LH-RH-like immunoreactivity has been demonstrated
in the hypothalamus of these vertebrates by radioimmunoassay
and immunocytochemistry (for reviews, see
Ball, 1981; Jackson, 1981; King and Millar, 1981). We have
shown that immunoreactive amphibian hypothalamic LH-RH
is identical with the mammalian decapeptide in chromatographic
properties and in its interaction with region-specific
LH-RH antisera, while immunoreactive LH-RHs from avian,
reptilian, and piscine hypothalami are structurally different
(King and Millar, 1979, 1980, 1981). These structural differences
of LH-RH-like peptides in submammalian vertebrates
have not been determined”
and:
“PMID: 6986260 [PubMed - indexed for MEDLINE]
King JA, Millar RP.
Immunoreactive LHRH was detected in hypothalamic and extrahypothalamic brain extracts of the rat, bird (pigeon and chicken), reptile (tortoise and lizard), amphibian (frog and toad), teleost (cichid), and elasmobranch (dogfish) and in the whole brain of the cyclostome (bagfish). The concentration of hypothalamic immunoreactive LHRH was more than 5-fold greater than that of the extrahypothalamic brain. Mammalian and amphibian hypothalamic immunoreactive LHRH yielded displacement curves parallel to those of synthetic LHRH in assays employing four antisera which recognize different regions of the decapeptide, thus suggesting a similarity in the structure of their LHRH. Hypothalamic immunoreactive LHRH from the bird, reptile, teleost, and elasmobranch differed from the mammalian and amphibian peptide in yielding displacement curves nonparallel to those of synthetic LHRH with three different antisera (1076, 743, and 744) which bind between Trp3 and Pro9 of LHRH. The differenece in structure appears to be near Leu7. With antiserum 422 which binds the NH2- and CO2H- termini of LHRH, bird, reptile, and teleost hypothalamic extracts yielded displacement curves parallel to that of synthetic LHRH. Bird, reptile, and teleost hypothalamic extracts showed displacement curves parallel to each other in all assays. In studies on the relative quantitation of LHRH, all four antisera gave similar values of immunoreactive LHRH concentration in mammalian hypothalamic extracts and in amphibian hypothalamic extracts. By contrast, assay of bird, reptile, teleost, and elasmobranch hypothalamic extracts with antiserum 422 gave much higher values than did the other antisera, suggesting that the LHRH peptide is structurally different from mammalian and amphibian LHRH in the region of Leu7 but similar at the NH2- and CO2H-termini. These conclusions are supported by studies on the biological activity of hypothalamic LHRH from the different species using dispersed ovine anterior pituitary cells in culture. The LH release responses to equivalent amounts of immunoreactive LHRH (as measured by antiserum 422 which binds a region of LHRH essential for biological activity) from the various species were similar, indicating that the biologically active region of the molecule has been conserved in evolution. Structural differences in vertebrate hypothalamic immunoreactive LHRH were confirmed by cation exchange and high pressure liquid chromatography. Our findings of differences and similarities in vertebrate LHRH support a contemporary phylogenetic scheme.”
The very things you complain about are the very things you do, that no one will engage you on a certain subject, that others have preformed opinions, that someone is not being “frank”, or would not be since you can't be bothered to actually ask the source.
So it's off you go to The Braying Chorus. Is that “frank” enough for you?
Except for everything I did offer. Like the fact that there are multiple LHRHs, BUT THEY DIDN'T KNOW THAT THEN, and that they don't even call them LHRHs in current literature and nomenclature.
Why do I always have to repeat everything three times with you? (And why am I so damn patient about it, even while being griped at and insulted? Just good natured, I guess.)
Oh, and that the first article you're giving me (and for which you don't provide a full cite for some reason)...
“Structure of Chicken Hypothalamic Luteinizing Hormone-releasing Hormone
II. ISOLATION AND CHARACTERIZATION*
(Received for publication, February 17, 1982)
Judy A. King and Robert P. Millar
... is not referenced anywhere in the "Sean D. Pitman M.D." article which CottShop linked. It's the same authors as "Comparative Aspects of Luteinizing Hormone-Releasing Hormone Structure and Function in Vertebrate Phylogeny," but published two years later.
Still, even it makes the point fabulously about being outdated:
The decapeptide LH-RH’ (pGlu-His-Trp-Ser-Tyr-Gly-Leu- Arg-Pro-Gly-NH 2 ), originally isolated from porcine hypothalami (Matsuo et al., 1971a) and later synthesized (Matsuo et al., 1971b), appears to have lower gonadotropin-releasing activity in submammalian vertebrates than in mammals.
Did you notice this CONTRADICTS the finding in their 1980 paper, wherein they inferred (by indirect immunological assay) that human and amphibian LHRH are more similar? Now they find the isolated and synthesized pig LHRH (the only pure isolate they seem to have) is LESS active in submammals.
Crude hypothalamic extracts from birds, reptiles, amphibians, and fish, however, contain significant gonadotropin-releasing activity, and LH-RH-like immunoreactivity has been demonstrated in the hypothalamus of these vertebrates
They still haven't even isolated LHRH in these other ("submammalian") species, let alone done any sequencing, but are only using "crude hypothalamic extracts"!
Sorry, but can you read?
has been demonstrated in the hypothalamus of these vertebrates by radioimmunoassay and immunocytochemistry (for reviews, see Ball, 1981; Jackson, 1981; King and Millar, 1981).
Radioimmunoassay?
Immunocytochemistry?
Crude hypothalamic extracts?
Hello, Hello, HELLO?!
OUTDATED!!!
King JA, Millar RP. Immunoreactive LHRH was detected in hypothalamic and extrahypothalamic brain extracts of the rat, bird (pigeon and chicken), reptile (tortoise and lizard), amphibian (frog and toad), teleost (cichid), and elasmobranch (dogfish) and in the whole brain of the cyclostome (bagfish). The concentration of hypothalamic immunoreactive LHRH was more than 5-fold greater than that of the extrahypothalamic brain. Mammalian and amphibian hypothalamic immunoreactive LHRH yielded displacement curves parallel to those of synthetic LHRH in assays employing four antisera
Yeah, and there's the one that is referenced by Pitman, and which I've already read.
So what was your point? I am wrong in claiming this research is outdated? Well, again, can you read?
Sorry, I really don't understand on what basis you are contesting this. I don't see it at all. The (slightly) newer article you introduce just strengthens the point, since even just two years later the obsolete point Pitman dishonestly extracted had been undermined!