Yes, he very clearly did.
"On April 14, 1865, after the fall of Richmond rendered moot his kidnap scheme, Booth set in motion his final plan--one of assassination. Booth may have made the decision to kill the President after hearing Lincoln deliver a speech urging Negro suffrage, according to Booth's former friend, Louis Weichmann. Weichmann spoke of his viewing of the the President's speech with Booth:
"I had never seen Mr. Lincoln up close and I knew he was a tall man, however nothing could have prepared me for the sight of him. A long shadow did he have. And his arms, when at his sides, touched near his knees. Very professionally he said that there would never be any suffrage based on differences in the way people look. Upon this, Booth turned to the two of us and said, That means nigger citizenship. Now by God Ill put him through!
http://www.law.umkc.edu/faculty/projects/ftrials/lincolnconspiracy/booth.html
Three days later, Booth shot Lincoln.
Walt