I know that a step of the manufacturing process is to treat the E. coli extracts with an enzyme called DNAse which destroys any DNA present. I also know that the mRNA is further purified using chemical and physical processes. Lastly, I know that any fragments of DNA that survive the purification process will be destroyed pretty quickly once they are injected into the body, since the body has multiple systems to identify and destroy foreign DNA.
Do you know how many ORFs were in the mRNA injections?
There is one single ORF in the mRNA vaccine. That ORF is surrounded by instructions telling the ribosome where to begin and end manufacturing the protein encoded in that ORF.
What an ORF even is?
ORF stands for Open Reading Frame. This is the sequence which actually encodes the protein. It consists of a series of three-letter words called codons. Each codon stands for a specific amino acid which is added to the growing protein on the ribosome. The ORF starts with the word AUG, which codes for the amino acid methionine, and is part of what is called the Kozak signal which tells the ribosome where to start making a protein. And the ORF ends with one of three stop codons (UAA, UAG, and UGA) that tell the ribosome to open up and release the new protein and the mRNA.
An mRNA typically consists of a single ORF. Sometimes, an organism uses frame shift mechanisms to find another ORF in the mRNA, but this is atypical.
Or that the m in the Pfizer mRNA jab stands for modified not messenger?
No. The "m" in mRNA *always* stands for messenger. Only professional antivaxxers tell you that it can stand for something else. Since they know that their target audience has no idea what mRNA is, they can basically say anything and their targets will believe them.
I only use the scientific terminology. There is no actual scientific shorthand for "modified." When we specifically want to stress that a particular mRNA molecule is modified, we say the whole word. It's kind of pointless, anyway, since all mRNA molecules are modified. Modification of RNA is a natural component of how cells make mRNA.
As far as I can tell, professional antivaxxers scare people with common scientific terminology since they know that the people they are targeting have never heard the terminology before. I suggest that instead of letting the strange terminology scare you, you try to find out what it actually means when used by scientists. Understanding is the key to combatting fear.
The Pfizer-BioNTech COVID-19 vaccine (BNT162b2) uses modified nucleotides. Specifically, it incorporates N1-methylpseudouridine instead of uridine. This modification helps to enhance the stability of the mRNA and reduce the innate immune response against the mRNA, improving the efficiency and effectiveness of the vaccine. Thus modRNA.
“There have been concerns and discussions about the potential presence of residual DNA plasmids in mRNA vaccines like the Pfizer-BioNTech COVID-19 vaccine due to the synthesis and production process. These DNA plasmids are used as templates for in vitro transcription to produce the mRNA. Ideally, purification steps during manufacturing should remove these plasmids to undetectable levels. However, trace amounts may sometimes be present as a residual impurity.” - chat gpt
Fast ai search…
“There have been concerns and discussions about the potential presence of residual DNA plasmids in mRNA vaccines like the Pfizer-BioNTech COVID-19 vaccine due to the synthesis and production process. These DNA plasmids are used as templates for in vitro transcription to produce the mRNA. Ideally, purification steps during manufacturing should remove these plasmids to undetectable levels. However, trace amounts may sometimes be present as a residual impurity.” - chat gpt
Fast ai search…
Oh - any DNA impurities are encapsulated with the modified RNA in the patented fatty globule - protecting it. Further, double stranded DNA is much more stable than the modified RNA.
Multiple ORFs:
“Eleven small overlapping ORFs (27-87 residues long) were discovered using NCBI ORFfinder on the wild-type spike protein sequence, and eight small ORFs (26-52 residues long) were found to overlap the Pfizer BNT162b2 vaccine mRNA sequence. “
Source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860813/#:~:text=Eleven%20small%20overlapping%20ORFs%20(27,Pfizer%20BNT162b2%20vaccine%20mRNA%20sequence.
Are you sticking with Fauci’s lie that the origins of Covid are not from the wuhan lab that he funded to do gain of function research? (But instead bad meat in a nearby market.)
Just how invested are you in the fauci lies?