It ain't just the unaltered ones that have the attitude. And with "calico momma" of the pseudo-ferals mentioned up-thread, there were other females. The queen-mother was still "da boss".
The undisputed alpha/madrina of my cartel of 4 rescue/shelter cats is a big spayed tan-and charcoal tabby with white vest and paws called Lucia-she is 15 years old, fat at 17 lbs and doesn’t take any s*** off the others-not even my black male, Merlyn who has quite the dominant streak himself-when a mouse finds its way into the kitchen door, all 4 go into action for the chase-and-kill until the mouse is trapped and beaten-then the other 3 sit down and let Lucia finish the execution, which she appears to really enjoy-I am glad Lucia is only a pet housecat...