Absolutely. And civil disobedience comes in many forms.
Someone needs to take the “Anarchists cookbook” and make a new one called the “Patriot’s Cookbook” with some plans for this sort of thing.
One idea that has come up for civil disobedience of dealing with federal government agents is called “drunking”. When a federal agent comes to enforce some sort of draconian unconstitutional measure they are “detained” and made to drink copious amounts of alcohol, they are then taken out a remote stretch of highway with their car and thier car is driven off the road and into something like a tree or light pole at a slow speed (enough to dsiable the car) by someone dressed in protective crash gear then the drunken SOB is then placed behind the wheel and left in their car. A call is made to local law enforcement authorities on a burner phone that there is crashed car on route XYZ and the local cops now have a federal tyrant drunken and behind the wheel.
But that is one example of non-violent way of dealing with a federal “beer-o-crat” that is non-violent short of good old tar and feathering.
I know of an instance (and I wont go into the details) where Game and Fish rangers went into a remote area to make sure trappers were following the law. The two rangers never came out. The official reason was bears got them.