I thought E=MC2 is what happens in fission and fusion, the result of splitting or adding protons from an atom.
The gas in the car is chemical energy.
Even chemical reactions invoke E = mc2
http://www.newton.dep.anl.gov/askasci/chem03/chem03534.htm
http://johnquiggin.com/index.php/archives/2005/10/01/really/ <- better one
increasing the the kinetic E of anything, increases it's mass. Unless the speed is hugh, that extra mass is ignored.