DNA (mitochondrail DNA, which can only be associated with maternal lineage) was sampled over time and from skeletons capable of being evaluated, from across North America peoples, and over time.
And compared to mitochondrial DNA in Northern Europe, Northern Asia and elsewhere.
Longer story shorter is that all peoples among all "native americans" in North America shared mitochondrial DNA with Northern European and Northern Asian peoples (this is a vast oversimplification of the process, but the process that was done is scientifically reliable, and does define lineage), all in North America shared DNA with Northern European and Northern Asian, WHILE the ONLY people in North America who did not but who instead possess lineage shared with Southern and Central Asians are the Hopi.
The Hopi "tribe," therefore, are people who migrated almost certainly from South/Central America into southwestern North America, while all the rest (including the Seminoles in Southeastern North America) migrated from the North, originally from Northern Europe and Northern Asia.
Whether the Hopi have remained in their current location is another issue, since there's now evidence that proves the ongoing "wars" and brutalities, even, in our Southwest by peoples fighting out territories and resources in the southwest and central americas, with one another (however, those peoples all share DNA and do not share DNA with the northern groups, and were, thus, almost certainly limited by opposing interests to their "farthest northern" inhabitations of the Hopi in the southwest).
Thank you.