Boot into Safe Mode and then run your anti-spyware tools.
Booting into Safe Mode only loads necessary processes. You can't remove some spyware while their malicious processes are running. And, you can't kill the processes.
In some cases, even safe mode won't do it... In those cases, you either need to boot the system to a Barts PE CD, or remove the hard drive, and install it as a secondary drive in a known, CLEAN system, and run all the spyware and virus removal tools.
It can get really ugly.
Mark
How do I Boot into Safe Mode w/Internet Explorer? Thanks