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 A grainy 11-minute video carried Kenneth Bigley's plea
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The brother of British hostage Kenneth Bigley says the US has "sabotaged" his brother's release by refusing to free a detained woman scientist.
Paul Bigley told the BBC there had been "a shadow of light" when Iraqi ministers said the woman would go free.
But the US, which is holding the woman, ruled out any release, saying it would not give in to the kidnappers.
Kenneth Bigley was seen in a video appealing to UK Prime Minister Tony Blair to help save his life.
The Iraqi government insisted on Thursday that it would not release two female weapons scientists despite statements on Wednesday that it would.
They have already killed two American hostages.
The US says it is holding two female weapons scientists, Rihab Rashid Taha and Huda Salih Mahdi Ammash. The UK says it has no Iraqi women in its custody.
Meanwhile the Italian government has dismissed as "unreliable" claims that two Italian aid workers held hostage in Iraq since 7 September had been killed.
In a message posted on the internet, a group calling itself the Jihad Organisation said the women were killed because Italy had not withdrawn its forces from Iraq.
Nothing to negotiate
Paul Bigley told BBC Radio 4's Today programme there had been "a shadow of light in a big, long, dark, damp, filthy, cold tunnel" when it appeared Dr Taha would be freed.
"Now this has been sabotaged," he said.
Profile: Tawhid and Jihad
"The fact on the table now is that nobody has to negotiate with anybody about anything," he said.
"All the powers have to do now is allow the Iraqis to conduct their own internal affairs the way they should be doing."
UK Foreign Secretary Jack Straw said the hostage had been placed in an appalling situation by evil men, but this could not alter the UK's position.
'Torture'
The 11-minute video of Mr Bigley's plea was apparently made by the Tawhid and Jihad Group headed by al-Qaeda suspect Abu Musab al-Zarqawi.
In it the hostage appeals to Mr Blair, saying: "You are the only person on God's earth who can help me."
"I think this is possibly my last chance," he says, sobbing repeatedly.
"I don't want to die... Please free female prisoners held in Iraqi prisons."
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RIHAB RASHID TAHA
Nicknamed Dr Germ
Worked on Saddam Hussein's biological weapons programme
Accused of producing anthrax
Educated in UK's University of East Anglia
Surrendered in May 2003
Considered important target, but not on US most-wanted list
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Mr Bigley's relatives have urged the kidnappers "to be all merciful".
Iraqi Minister of State Kassem Daoud denied that his government had bowed to US pressure in deciding not to release Dr Taha.
The decision was made by interim Prime Minister Iyad Allawi in order to show that Iraq would not negotiate with the kidnappers, he told Today.
"Unfortunately... there was an impression that the Iraqi government made a deal with the terrorists, which we never, ever do," he said.
Mr Bigley, 62, was among three Western civilian contractors kidnapped a week ago in Baghdad.
Video footage released on Monday showed American Eugene Armstrong being killed by a masked man said by the CIA to be Mr Zarqawi.
A second American, Jack Hensley, was killed 24 hours later. A video purportedly showing his killing was posted on Wednesday evening after his body had been found.