Gen 1:27
27 So God created man in his own image, in the image of God created he him; male and female created he them.
Gen 2:23-24
23 And Adam said, This is now bone of my bones, and flesh of my flesh: she shall be called Woman, because she was taken out of Man.
24 Therefore shall a man leave his father and his mother, and shall cleave unto his wife: and they shall be one flesh.
Matthew 19:4-5
4 And he answered and said unto them, Have ye not read, that he which made them at the beginning made them male and female,
5 And said, For this cause shall a man leave father and mother, and shall cleave to his wife: and they twain shall be one flesh?
This also helps those who take issue with the supposed differing creation accounts in Gen Chapter 1 and Gen Chapter 2. The Holy Spirit has it all covered for our admonition. We worship an awesome God who foresees these issues.
Jesus' recorded words:
"Have ye not read, that he which made them at the beginning made them male and female,"
Since Adam and Eve logicly speaking, were the only two made by God's own hand and their being made "at the beginning", it's not too difficult to conclude Jesus's reference to Adam and Eve as "them", specifically.