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To: AndrewC
I have also looked at fumarase and 40S ribosomal protein S4. These do not change much. The ribosomal protein shows that the chicken is closer to human than mammals outside of primates.(in terms of that protein)

Well, I warned you about canned programs. The human protein has an extra N-terminal and C-terminal that most mammals lack (you can count each deletion as a single mutation, if you like), but if you focus on the common sequence, I get 4 differences between Sus scrofa (pig; go figure) and H. sapiens; and 12 between Gallus gallus and H. sapiens. Bos taurus is almost identical to S. scrofa (1 change)

My chicken is the chicken rps4 (P47836); human is the Y-linked rps4 (AAH10286) and the pig is BAA21081; pigs only have one sex-chromosome linked rps4. Cow is BAA21078.

I can post the aligned sequences on a web page., if anyone wants to see for himself.

593 posted on 03/14/2003 12:53:36 PM PST by Right Wing Professor
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To: Right Wing Professor
How does (( evolution )) ...

spontaneous (( magic )) -- morphing (( flux )) ...

matter and life ... constitution (( living )) become SCIENCE (( law )) ---

silly isn't it !

Liberal social engineering (( revisionism // fantasy )) ! !



594 posted on 03/14/2003 1:16:52 PM PST by f.Christian (( + God =Truth + love courage // LIBERTY logic + SANITY + Awakening + ))
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To: Right Wing Professor
chicken placemarker
596 posted on 03/14/2003 1:25:42 PM PST by js1138
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To: Right Wing Professor
Well, I warned you about canned programs.

Well, here is what 2 way blast shows about your comparisons. Note: the chicken protein is the same length as the human protein.

Sequence 1 gi 16307477 ribosomal protein S4, Y-linked [Homo sapiens] Length 263 (1 .. 263)
Sequence 2 gi 1350995 40S ribosomal protein S4. This is the chicken -- Andy Length 263 (1 .. 263)

NOTE:The statistics (bitscore and expect value) is calculated based on the size of nr database

Score =  511 bits (1317), Expect = e-144
Identities = 247/263 (93%), Positives = 254/263 (95%)




Query:          1   MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDE 60
                    MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLI+FLRNRLKYALTGDE
Sbjct:          1   MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDE 60
S4 RNA-BINDING. 42                                           *******************
RPS4            1   ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Query:          61  VKKICMQRFIKIDGKVRVDVTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAK 120
                    VKKICMQRFIKIDGKVR D+TYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRIT EEAK
Sbjct:          61  VKKICMQRFIKIDGKVRTDITYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITAEEAK 120
S4 RNA-BINDING. 61  ********************************************
RPS4            61  ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Query:          121 YKLCKVRKITVGVKGIPHLVTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNL 180
                    YKLCKVRKI VG KGIPHLVTHDARTIRYPDP+IKVNDT+QIDL TGKI +FIKFDTGNL
Sbjct:          121 YKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNL 180
RPS4            121 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Query:          181 CMVIGGANLGRVGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGNGNKPWISLPR 240
                    CMV GGANLGR+GVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIG GNKPWISLPR
Sbjct:          181 CMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPR 240
RPS4            181 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Query:          241 GKGIRLTVAEERDKRLATKQSSG 263
                    GKGIRLT+AEERDKRLA KQSSG
Sbjct:          241 GKGIRLTIAEERDKRLAAKQSSG 263
RPS4            241 +++++++++++++++++++++++
Sequence 1 gi 16307477 ribosomal protein S4, Y-linked [Homo sapiens] Length 263 (1 .. 263)
Sequence 2 gi 4432942 ribosomal protein S4 [Sus scrofa] Length 194 (1 .. 194)

NOTE:The statistics (bitscore and expect value) is calculated based on the size of nr database

Score =  371 bits (953), Expect = e-102
Identities = 178/194 (91%), Positives = 187/194 (95%)




Query: 20  LDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVD 79
           LDKLTGVFAPRPSTGPHKLRECLPLI+FLRNRLKYALTGDEVKKICMQRFIKIDGKVR D
Sbjct: 1   LDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTD 60

Query: 80  VTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHL 139
           +TYPAGFMDVISI+KTGE+FRL+YDTKGRFAVHRIT EEAKYKLCKVRKI VG KGIPHL
Sbjct: 61  ITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHL 120

Query: 140 VTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRE 199
           VTHDARTIRYPDP+IKVNDT+QIDL TGKI +FIKFDTGNLCMV GGANLGR+GVITNRE
Sbjct: 121 VTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRE 180

Query: 200 RHPGSFDVVHVKDA 213
           RHPGSFDVVHVKDA
Sbjct: 181 RHPGSFDVVHVKDA 194
Sequence 1 gi 4432939 ribosomal protein S4 [Bos taurus] Length 194 (1 .. 194)
Sequence 2 gi 4432942 ribosomal protein S4 [Sus scrofa] Length 194 (1 .. 194)

NOTE:The statistics (bitscore and expect value) is calculated based on the size of nr database

Score =  395 bits (1014), Expect = e-109
Identities = 192/194 (98%), Positives = 193/194 (98%)




Query: 1   LDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTD 60
           LDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTD
Sbjct: 1   LDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTD 60

Query: 61  VTYPAGFMDVISIDKIGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHL 120
           +TYPAGFMDVISIDK GENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHL
Sbjct: 61  ITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHL 120

Query: 121 VTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRE 180
           VTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRE
Sbjct: 121 VTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRE 180

Query: 181 RHPGSFDVVHVKDA 194
           RHPGSFDVVHVKDA
Sbjct: 181 RHPGSFDVVHVKDA 194

Here I grabbed the pieces from the chicken and the human protein in order to compare the same range as the pig. I think that this is not the correct way to analyze the data but as you can see the chicken still wins by 181 to 178.

Sequence 1 lcl|seq_1 This is chicken -- Andy Length 194 (1 .. 194)
Sequence 2 lcl|seq_2 This is human -- Andy Length 194 (1 .. 194)

NOTE:The statistics (bitscore and expect value) is calculated based on the size of nr database

Score =  376 bits (966), Expect = e-103
Identities = 181/194 (93%), Positives = 187/194 (96%)




Query: 1   LDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTD 60
           LDKLTGVFAPRPSTGPHKLRECLPLI+FLRNRLKYALTGDEVKKICMQRFIKIDGKVR D
Sbjct: 1   LDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVD 60

Query: 61  ITYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITAEEAKYKLCKVRKIFVGTKGIPHL 120
           +TYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRIT EEAKYKLCKVRKI VG KGIPHL
Sbjct: 61  VTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHL 120

Query: 121 VTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRE 180
           VTHDARTIRYPDP+IKVNDT+QIDL TGKI +FIKFDTGNLCMV GGANLGR+GVITNRE
Sbjct: 121 VTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRE 180

Query: 181 RHPGSFDVVHVKDA 194
           RHPGSFDVVHVKDA
Sbjct: 181 RHPGSFDVVHVKDA 194

601 posted on 03/14/2003 1:47:30 PM PST by AndrewC
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