Ivermectin has New Application in Inhibiting Colorectal Cancer Cell Growth

Frontiers in Pharmacology ^ | 10/21/2021 | Research from Infection and Immunity Institute and Translational Medical Center of Huaihe Hospital

[SeekAndFind]

Colorectal cancer (CRC) is the third most common cancer worldwide and still lacks effective therapy. Ivermectin, an antiparasitic drug, has been shown to possess anti-inflammation, anti-virus, and antitumor properties. However, whether ivermectin affects CRC is still unclear. The objective of this study was to evaluate the influence of ivermectin on CRC using CRC cell lines SW480 and SW1116. We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, used Annexin V-FITC/7-AAD kit to determine cell apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell cycle. The results demonstrated that ivermectin dose-dependently inhibited colorectal cancer SW480 and SW1116 cell growth, followed by promoting cell apoptosis and increasing Caspase-3/7 activity. Besides, ivermectin upregulated the expression of proapoptotic proteins Bax and cleaved PARP and downregulated antiapoptotic protein Bcl-2. Mechanism analysis showed that ivermectin promoted both total and mitochondrial ROS production in a dose-dependent manner, which could be eliminated by administering N-acetyl-l-cysteine (NAC) in CRC cells. Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (2.5 and 5 µM) induced CRC cell arrest.

Overall, ivermectin suppressed cell proliferation by promoting ROS-mediated mitochondrial apoptosis pathway and inducing S phase arrest in CRC cells, suggesting that ivermectin might be a new potential anticancer drug therapy for human colorectal cancer and other cancers.

THIS IS A SNIPPET. CLICK ON ABOVE ARTICLE LINK FOR THE RESEARCH DETAILS

Results

Ivermectin Inhibits the Proliferation of Colorectal Cancer Cells

To explore the effect of ivermectin on the proliferation of colorectal cancer cells, we used different concentrations of ivermectin (0, 2.5, 5, 10, 15, 20, and 30 μM) to culture colorectal cancer cells SW480 and SW1116. CCK-8 assay was performed to measure SW480 and SW1116 cancer cell proliferation after cells were incubated for 12, 24, and 36 h. As shown in Figure 2, the cell viability of SW480 and SW1116 cells decreased dose-dependently by ivermectin treatment (Dose, D: p < 0.01).

Furthermore, ivermectin inhibited SW480 and SW1116 cell viability in a time-dependent manner (Time, T: p < 0.01). Finally, Table 1 showed that the IC50 of SW480 cells treated with ivermectin for 12, 24, and 36 h was 16.17 ± 0.76 μM, 15.34 ± 0.81 μM, and 12.11 ± 0.97 μM, respectively; and the IC50 of SW1116 cells treated with ivermectin for 12, 24, and 36 h were 7.60 ± 0.62 μM, 6.27 ± 0.70 μM and 5.76 ± 0.81 μM, respectively. The present data indicate that ivermectin might have more sensitive to SW1116 cells than that of SW480 cells.

[Norski]

If information accurate, not surprising.

Was not aware of it until about ten years ago, but there is apparently a link between parasite infestation and cancer.

Naturopath and researcher Dr. Hulda Clark wrote extensively on it.

[Baldwin77]

Interesting

[thinden]

Bttt

[BobL]

Note that they NEVER say whether this study was done on humans, or horses.

[Adder]

Nope...can’t use it...its a horse dewormer for God’s sakes...and yall ain’t horses...

[Babba Gi]

Ivermectin cures sticky valves, clogged lifters and removes sludge.

[ryderann]

Don’t forget about Ivermectin’s use for Lyme disease.

[9YearLurker]

That is interesting.

[TexasCruzin]

Fenbendazole is also a very powerful anticancer drug. It save my Niece Sherry from state 4 breast cancer which spread to her lungs. She’s cancer free now. Read: Mycancerstory.rocks

[Hiddigeigei]

"different concentrations of ivermectin (0, 2.5, 5, 10, 15, 20, and 30 μM)"

Interesting graphs, but I'm having a little trouble understanding it. Isn't μM (micron?) a measurement of length? How does it convert to dosage?

[Delta 21]

Not so but IVM is probably gonna cost you more.

https://www.pushhealth.com/drugs/ivermectin hooked me up with an in state authorized prescriber, RX to my local pharmacy and $4 a pill later my covid kit is complete.

You can find a DR to do a chop~yer~dick~off~me operation and pay cash to do it because insurance wont cover it...The government used the cells from aborted babies to make the medicine it want you to take then shames you into fear that only thir way works??!?? well, Pursue getting the IVM and saving your life just as enthusiastically as the freak wanting to self mutilate!

DO IT !!

[Oorang]

Interesting that NAC reversed ivermectin’s inhibiting effect.

I read the article at the link but not being a scientist not a lot made sense to me. So, is the study saying that NAC negates the cancer cell killing effect brought on by the Ivermectin or is it saying the the Ivermectin kills the cancer cells and the NAC cleans the dead cells out?
Thanks for any clarification.

[FreedomForce]

“Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed.”

NAC negates the cancer killing effect brought on by the ivermectin is what I understand the study to mean.