Ivermectin has New Application in Inhibiting Colorectal Cancer Cell Growth

Frontiers in Pharmacology ^ | 10/21/2021 | Research from Infection and Immunity Institute and Translational Medical Center of Huaihe Hospital

[SeekAndFind]

Colorectal cancer (CRC) is the third most common cancer worldwide and still lacks effective therapy. Ivermectin, an antiparasitic drug, has been shown to possess anti-inflammation, anti-virus, and antitumor properties. However, whether ivermectin affects CRC is still unclear. The objective of this study was to evaluate the influence of ivermectin on CRC using CRC cell lines SW480 and SW1116. We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, used Annexin V-FITC/7-AAD kit to determine cell apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell cycle. The results demonstrated that ivermectin dose-dependently inhibited colorectal cancer SW480 and SW1116 cell growth, followed by promoting cell apoptosis and increasing Caspase-3/7 activity. Besides, ivermectin upregulated the expression of proapoptotic proteins Bax and cleaved PARP and downregulated antiapoptotic protein Bcl-2. Mechanism analysis showed that ivermectin promoted both total and mitochondrial ROS production in a dose-dependent manner, which could be eliminated by administering N-acetyl-l-cysteine (NAC) in CRC cells. Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (2.5 and 5 µM) induced CRC cell arrest.

Overall, ivermectin suppressed cell proliferation by promoting ROS-mediated mitochondrial apoptosis pathway and inducing S phase arrest in CRC cells, suggesting that ivermectin might be a new potential anticancer drug therapy for human colorectal cancer and other cancers.

THIS IS A SNIPPET. CLICK ON ABOVE ARTICLE LINK FOR THE RESEARCH DETAILS

Results

Ivermectin Inhibits the Proliferation of Colorectal Cancer Cells

To explore the effect of ivermectin on the proliferation of colorectal cancer cells, we used different concentrations of ivermectin (0, 2.5, 5, 10, 15, 20, and 30 μM) to culture colorectal cancer cells SW480 and SW1116. CCK-8 assay was performed to measure SW480 and SW1116 cancer cell proliferation after cells were incubated for 12, 24, and 36 h. As shown in Figure 2, the cell viability of SW480 and SW1116 cells decreased dose-dependently by ivermectin treatment (Dose, D: p < 0.01).

Furthermore, ivermectin inhibited SW480 and SW1116 cell viability in a time-dependent manner (Time, T: p < 0.01). Finally, Table 1 showed that the IC50 of SW480 cells treated with ivermectin for 12, 24, and 36 h was 16.17 ± 0.76 μM, 15.34 ± 0.81 μM, and 12.11 ± 0.97 μM, respectively; and the IC50 of SW1116 cells treated with ivermectin for 12, 24, and 36 h were 7.60 ± 0.62 μM, 6.27 ± 0.70 μM and 5.76 ± 0.81 μM, respectively. The present data indicate that ivermectin might have more sensitive to SW1116 cells than that of SW480 cells.

[SeekAndFind]

Ivermectin inhibits cell proliferation of SW480 and SW1116 cells. SW480 (A) and SW1116 cells (B) were cultured at different concentrations of ivermectin (0, 2.5, 5, 10, 15, 20, and 30 μM) for 12, 24, and 36 h, and then CCK-8 assay was performed to detect the cell proliferation. The experiment was repeated three times, and data were presented as mean ± S.E.M. The interaction between dose (D) and time (T) effect were analyzed using two-way ANOVA following Tukey’s t-test. D: dose effect; T: time effect: T × D: the interaction between time and dose effect.

[SeekAndFind]

Inhibitory concentration (IC50) of IVM on the viability of colorectal cells.

[Oscar in Batangas]

According to “some” VERY BRIGHT EXPERTS, this will only work if you are a horse or goat.
Sarc off

[bray]

Ivermectin may be the wonder drug.

[The Truth Will Make You Free]

Three Great Drugs in History: Penicillin, Aspirin & Ivermectin

[FreedomForce]

Interesting that NAC reversed ivermectin’s inhibiting effect.

[neverbluffer]

Ivermectin is similiar to Fenbenzadole. Both are anti parasitic drugs.

Read Joe Tippins story about his cancer which gave him 3 months to live and he survived with Fenbenzadole. Now we hear Ivermectin can help with colorectal cancer. There is something to it.

[Secret Agent Man]

oh wait though

ivermectin sucks

and its a horse dewormer

these people are such g-d hypocrites

[WildHighlander57]

From the article:

“Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (2.5 and 5 µM) induced CRC cell arrest.”

This says N-Acetyl-Cysteine defeats Ivermectin’s cancer- fighting abilities.

Question:

Does it do the same thing to Ivermectin’s covid-fighting abilities?

Ping!!

[bray]

It is easier to get heroin than IVM.

[pepsi_junkie]

Great news for all those horses with cancer! The FDA won’t let anyone else use it, of course. Not politically palatable.

[Trillian]

Interesting.

[Bob434]

Someone wrote the other day “Aspirin comes from willow bark- when you take aspirin, you are eating wood, seriously ya’ll, just stop it’ lol

[Tellurian]

Does (NAC) (defeat) Ivermectin’s covid-fighting abilities

dog-ear for later

[UnwashedPeasant]

Reactive oxygen species (ROS) — These are free radicals. We take antioxidants to get rid of these.

If ivermectin makes these, I hope they are only in cancer cells. Otherwise, it might be better to use ivermectin for treatment and not for prevention.

[steve86]

I happened to take it today for suspicious respiratory symptoms. Good to know the other applications ..

[steve86]

Good catch. Well, I won’t take NAC on the same day as ivermectin. NAC’s useful life in the body is very short...hours at most.

[steve86]

More to the point to say if you’re taking ivermectin for cancer fighting....don’t take NAC.

And if you take ivermectin at beginning of covid symptoms you probably won’t need NAC for lung protection.

[tired&retired]

Thanks for posting..