No. A pardon means no punishment or other 'disability' may be imposed on the person pardoned. Period.
A number of court rulings indicate using a pardon in court as a defense is an admission of guilt, notably Burdick v. United States.
Constitutional Convention on Monday, August 27, 1787,
Mr. L. MARTIN moved to insert the words ‘after conviction’ after the words ‘reprieves and pardons.’
Mr. WILSON objected that pardon before conviction might be necessary to obtain the testimony of accomplices. He stated the case of forgeries in which this might particularly happen.
Mr. L. MARTIN withdrew his motion.
Burdick reversed this deliberate decision by the authors of the constitution.